Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Srivastava, S.K.; Dube, D.; Kukshal, V.; Jha, A.K.; Hajela, K.; Ramachandran, R.
    NAD+-dependent DNA ligase (Rv3014c) from Mycobacterium tuberculosis: novel structure-function relationship and identification of a specific inhibitor (2007), Proteins, 69, 97-111.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.2 additional information deletion of just the BRCT domain from MtuLigA results in total loss of activity in in vitro assays Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.2 12-(6-piperidin-1-ylhexyl)-7,12-dihydro-6H-[1]benzothiepino[5,4-b]indole
-
 Mycobacterium tuberculosis
6.5.1.2 N4-(7-chloroquinolin-4-yl)-N1,N1-diethylpentame-1,4-diamine
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.2 0.00146
-
 NAD+
-
 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.2 Ca2+ in presence of Ca2+ MtuLigA is able to carry out the first two steps of ligation reaction that is the transfer of AMP to enzyme from NAD+ and further transfer of it to substrate to form DNA-adenylate intermediate. Ca2+ can not support nick closure activity Mycobacterium tuberculosis
6.5.1.2 Mg2+ divalent cation required, optimal concentration: 10 mM Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Mycobacterium tuberculosis P9WNV1
-
-
6.5.1.2 Mycobacterium tuberculosis H37Rv P9WNV1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Mycobacterium tuberculosis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Mycobacterium tuberculosis H37Rv AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.2 MtuLigA
-
 Mycobacterium tuberculosis
6.5.1.2 NAD+-dependent DNA ligase
-
 Mycobacterium tuberculosis
6.5.1.2 Rv3014c
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.2 8
-
-
 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.2 6.5 9.5 more than 60% of maximal activity between pH 7.5 and 8.5 Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.5.1.2 0.0135
-
-
 Mycobacterium tuberculosis 12-(6-piperidin-1-ylhexyl)-7,12-dihydro-6H-[1]benzothiepino[5,4-b]indole
6.5.1.2 0.046
-
-
 Mycobacterium tuberculosis N4-(7-chloroquinolin-4-yl)-N1,N1-diethylpentame-1,4-diamine