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Literature summary extracted from

  • Shi, D.; Yu, X.; Roth, L.; Morizono, H.; Tuchman, M.; Allewell, N.M.
    Structures of N-acetylornithine transcarbamoylase from Xanthomonas campestris complexed with substrates and substrate analogs imply mechanisms for substrate binding and catalysis (2006), Proteins, 64, 532-542.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.9 purified recombinant enzyme in binary complex with its substrates, carbamoyl phosphate or N-acetyl-L-ornithine, and in ternary complex with carbamoyl phosphate and N-acetyl-L-norvaline, hanging drop vapour diffusion method, preparation by mixing of 0.002 ml of 12 mg/ml protein solution with an equal volume of reservoir solution containing the ligands, cryoprotection, X-ray diffraction structure determination and analysis at 1.8-1.95 A resolution, modeling of the transition state complex Xanthomonas campestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.9 carbamoyl phosphate + N2-acetyl-L-ornithine Xanthomonas campestris
-
phosphate + N-acetyl-L-citrulline
-
?
2.1.3.9 additional information Xanthomonas campestris the enzyme is essential in the arginine biosynthesis in several eubacteria ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.9 Xanthomonas campestris
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.9 carbamoyl phosphate + N2-acetyl-L-ornithine = phosphate + N-acetyl-L-citrulline substrate binding and catalytic mechanism, modeling of the transition state complex Xanthomonas campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.9 carbamoyl phosphate + N2-acetyl-L-ornithine
-
Xanthomonas campestris phosphate + N-acetyl-L-citrulline
-
?
2.1.3.9 additional information the enzyme is essential in the arginine biosynthesis in several eubacteria Xanthomonas campestris ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.3.9 AOTCase
-
Xanthomonas campestris
2.1.3.9 More the enzyme is a member of the transcarbamoylase superfamily Xanthomonas campestris
2.1.3.9 N-acetylornithine transcarbamoylase
-
Xanthomonas campestris