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Literature summary extracted from

  • Rehse, P.H.; Tahirov, T.H.
    Crystal structure of a purine/pyrimidine phosphoribosyltransferase-related protein from Thermus thermophilus HB8 (2005), Proteins, 61, 658-665.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.7 expression of wild-type enzyme in Escherichia coli strain BL21(DE3) and of the selenomethionine-labeled enzyme in Escherichia coli strain B834 Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.7 purified recombinant native and selenomethionine-labeled enzymes, sitting drop vapour diffusion method, 0.001 ml of 20 mg/ml native protein in 20 mM Tris-HCl, 50 mM NaCl, pH 8.0, is mixed with an equal volume of reservoir solution containing 100 mM MES, pH 5.5, 100 mM calcium acetate, 3% w/v PEG 10000, and 3% v/v MeOH, 1 week, for the selenomethionine-labeled protein 40 mM calcium acetate, 1.5% w/v PEG 10000, and 3% v/v MeOH is used at a protein concentration of 29.4 mg/ml, X-ray diffraction structure determination and analysis at 1.9-2.6 A resolution, multiple wavelength anomalous dispersion method, asymmetric unit of two pairs of identical dimers, each related by noncrystallographic two-fold symmetry, a fifth monomer forms a similar dimer across a crystallographic two-fold axis, modeling Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.7 5-phospho-alpha-D-ribosyl-1-diphosphate + adenine Thermus thermophilus the enzyme is involved in the salvage of adenosine, overview AMP + diphosphate
-
 ?
2.4.2.7 5-phospho-alpha-D-ribosyl-1-diphosphate + adenine Thermus thermophilus HB8 / ATCC 27634 / DSM 579 the enzyme is involved in the salvage of adenosine, overview AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Thermus thermophilus
-
-
-
2.4.2.7 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7 recombinant wild-type enzyme from Escherichia coli strain BL21(DE3) and recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834 by two steps of anion exchange chromatography, and gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 5-phospho-alpha-D-ribosyl-1-diphosphate + adenine
-
 Thermus thermophilus AMP + diphosphate
-
 ?
2.4.2.7 5-phospho-alpha-D-ribosyl-1-diphosphate + adenine the enzyme is involved in the salvage of adenosine, overview Thermus thermophilus AMP + diphosphate
-
 ?
2.4.2.7 5-phospho-alpha-D-ribosyl-1-diphosphate + adenine
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate
-
 ?
2.4.2.7 5-phospho-alpha-D-ribosyl-1-diphosphate + adenine the enzyme is involved in the salvage of adenosine, overview Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.7 adenine phosphoribosyltransferase
-
 Thermus thermophilus
2.4.2.7 APRTase
-
 Thermus thermophilus