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Literature summary extracted from

  • Runquist, J.A.; Miziorko, H.M.
    Functional contribution of a conserved, mobile loop histidine of phosphoribulokinase (2006), Protein Sci., 15, 837-842.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.19 expressed in Escherichia coli strain BL21(DE3) Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.19 H100A reduced activity Cereibacter sphaeroides
2.7.1.19 H100N reduced activity Cereibacter sphaeroides
2.7.1.19 H100Q reduced activity Cereibacter sphaeroides
2.7.1.19 H45N similar to wild type enzyme Cereibacter sphaeroides
2.7.1.19 Y98L reduced activity Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.19 6-phosphogluconate competitive inhibitor, 78% residual activity at 1 mM Cereibacter sphaeroides
2.7.1.19 diethyl dicarbonate 27% residual activity at 0.04 mM Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.19 0.1
-
 D-ribulose 5-phosphate wild type enzyme, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate Cereibacter sphaeroides
2.7.1.19 0.78
-
 D-ribulose 5-phosphate mutant enzyme H100A, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate Cereibacter sphaeroides
2.7.1.19 1.35
-
 D-ribulose 5-phosphate mutant enzyme H100Q, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate Cereibacter sphaeroides
2.7.1.19 1.37
-
 D-ribulose 5-phosphate mutant enzyme H100N, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate Cereibacter sphaeroides
2.7.1.19 4
-
 D-ribulose 5-phosphate mutant enzyme Y98L, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.19 Q Sepharose chromatography Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP
-
 Cereibacter sphaeroides D-ribulose 1,5-diphosphate + ADP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.19 Prk
-
 Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.19 ATP
-
 Cereibacter sphaeroides