Literature summary extracted from
Li, X.; Guo, M.; Fan, J.; Tang, W.; Wang, D.; Ge, H.; Rong, H.; Teng, M.; Niu, L.; Liu, Q.; Hao, Q.
Crystal structure of 3-hydroxyanthranilic acid 3,4-dioxygenase from Saccharomyces cerevisiae: a special subgroup of the type III extradiol dioxygenases (2006), Protein Sci., 15, 761-773.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.13.11.6 |
Y3HAO protein is subcloned into the pET-28a expression vector from extracted Saccharomyces cerevisiae genomic DNA, and the Y3HAO protein is highly expressed as a soluble protein in Escherichia coli strain BL21(DE3) with a six-residueHis tag attached to its N-terminus |
Saccharomyces cerevisiae |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.13.11.6 |
hanging drop-vapor diffusion method, 2.4 A resolution |
Saccharomyces cerevisiae |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.13.11.6 |
Fe2+ |
non-heme ferrous enzyme |
Saccharomyces cerevisiae |
|
1.13.11.6 |
Ni2+ |
two nickel binding sites per molecule. One of the bound nickel atoms occupies the proposed ferrous-coordinated active site |
Saccharomyces cerevisiae |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.13.11.6 |
3-hydroxyanthranilate + O2 |
Saccharomyces cerevisiae |
in the kynurenine pathway |
2-amino-3-carboxymuconate semialdehyde |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.11.6 |
Saccharomyces cerevisiae |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.13.11.6 |
recombinant |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.11.6 |
3-hydroxyanthranilate + O2 |
in the kynurenine pathway |
Saccharomyces cerevisiae |
2-amino-3-carboxymuconate semialdehyde |
- |
? |
|
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.13.11.6 |
additional information |
non-heme enzyme |
Saccharomyces cerevisiae |
|