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Literature summary extracted from

  • Webster, L.T.; Gerowin, L.D.; Rakita, L.
    Purification and characteristics of a butyryl coenzyme A synthetase from bovine heart mitochondria (1965), J. Biol. Chem., 240, 29-33.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.2 (NH4)2SO4
-
 Bos taurus
6.2.1.2 p-chloromercuribenzoate butyrate or ATP partially protects Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.2 0.85
-
 CoA
-
 Bos taurus
6.2.1.2 1.5
-
 Butyrate
-
 Bos taurus
6.2.1.2 3
-
 ATP
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.2 mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.2 Mg2+ required Bos taurus
6.2.1.2 Mg2+ Km: 1.8 mM Bos taurus
6.2.1.2 Mn2+ can replace Mg2+ in activation Bos taurus
6.2.1.2 additional information no differential response to monovalent cations Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.2 heart
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.2 3.3
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.2 ATP + butyrate + CoA no activity with GTP Bos taurus AMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.2 ATP + crotonate + CoA i.e. E-2-butenoate, 20% of the activity relative to butyrate Bos taurus AMP + diphosphate + crotonyl-CoA
-
 ?
6.2.1.2 ATP + isobutyrate + CoA 20% of the activity relative to butyrate Bos taurus AMP + diphosphate + isobutyryl-CoA
-
 ?
6.2.1.2 CTP + butyrate + CoA slight activity Bos taurus CMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.2 ITP + butyrate + CoA slight activity Bos taurus IMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.2 additional information no activity with formate and heptanoate Bos taurus ?
-
 ?
6.2.1.2 additional information straight chain acids having 3, 5 or 6 carbons are less active as substrates than butyrate Bos taurus ?
-
 ?
6.2.1.2 additional information no activity with acetate and octanoate Bos taurus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.2 8
-
 broad pH-optimum around Bos taurus