EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.3.3 | cloning of cDNAs into pMAL plasmid and expression in Escherichia coli as a fusion protein with MBP tag or hexameric His tag | Sabellastarte spectabilis |
2.7.3.3 | expressed in Escherichia coli | Sabellastarte spectabilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.3.3 | 4.2 | - |
L-Arg | the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 13.9 | - |
D-Arg | the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 34.3 | - |
L-Arg | 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 35.4 | - |
L-Arg | 25°C | Sabellastarte spectabilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.3.3 | 40000 | - |
2 * 40000, Superdex 75 gel filtration | Sabellastarte spectabilis |
2.7.3.3 | 42273 | - |
2 * 42273, calculated from sequence | Sabellastarte spectabilis |
2.7.3.3 | 42887 | - |
2 * 42887, calculated from sequence | Sabellastarte spectabilis |
2.7.3.3 | 42890 | - |
calculated from sequence of cDNA | Sabellastarte spectabilis |
2.7.3.3 | 80000 | - |
fusion proteins with hexameric His tag, gel filtration | Sabellastarte spectabilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.3.3 | ATP + D-Arg | Sabellastarte spectabilis | - |
ADP + omega-N-phospho-D-Arg | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.3.3 | Sabellastarte spectabilis | - |
- |
- |
2.7.3.3 | Sabellastarte spectabilis | Q4AED1 | - |
- |
2.7.3.3 | Sabellastarte spectabilis | Q4AED2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.3.3 | Amberlite IR120BNA column chromatography and reverse-phase high performance liquid chromatography | Sabellastarte spectabilis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.3.3 | body wall muscle | - |
Sabellastarte spectabilis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.3.3 | ATP + D-Arg | 35.2% relative activity compared to L-Arg | Sabellastarte spectabilis | ADP + Nomega-phospho-D-Arg | - |
? | |
2.7.3.3 | ATP + D-Arg | 35.2% of the activity with L-Arg | Sabellastarte spectabilis | ADP + Nomega-phospho-D-Arg | - |
? | |
2.7.3.3 | ATP + D-Arg | - |
Sabellastarte spectabilis | ADP + omega-N-phospho-D-Arg | - |
? | |
2.7.3.3 | ATP + D-Arg | 7.6% of the activity with L-Arg | Sabellastarte spectabilis | ADP + omega-N-phospho-D-Arg | - |
? | |
2.7.3.3 | ATP + L-Arg | - |
Sabellastarte spectabilis | ADP + Nomega-phospho-L-Arg | - |
? | |
2.7.3.3 | ATP + L-Arg | 100% activity | Sabellastarte spectabilis | ADP + Nomega-phospho-L-Arg | - |
? | |
2.7.3.3 | ATP + L-Arg | - |
Sabellastarte spectabilis | ADP + omega-N-phospho-L-Arg | - |
? | |
2.7.3.3 | ATP + lombricine | 0.17% of the activity with L-Arg | Sabellastarte spectabilis | ADP + omega-N-phospholombricine | - |
? | |
2.7.3.3 | ATP + lombricine | 3.0% of the activity with L-Arg | Sabellastarte spectabilis | ADP + omega-N-phospholombricine | - |
? | |
2.7.3.3 | ATP + taurocyamine | 0.11% of the activity with L-Arg | Sabellastarte spectabilis | ADP + N-phosphotaurocyamine | - |
? | |
2.7.3.3 | ATP + taurocyamine | 2.7% of the activity with L-Arg | Sabellastarte spectabilis | ADP + N-phosphotaurocyamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.3.3 | dimer | 2 * 40000, Superdex 75 gel filtration | Sabellastarte spectabilis |
2.7.3.3 | dimer | 2 * 42273, calculated from sequence | Sabellastarte spectabilis |
2.7.3.3 | dimer | 2 * 42887, calculated from sequence | Sabellastarte spectabilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.3.3 | AK1 | - |
Sabellastarte spectabilis |
2.7.3.3 | AK2 | - |
Sabellastarte spectabilis |
2.7.3.3 | arginine kinase 1 | - |
Sabellastarte spectabilis |
2.7.3.3 | arginine kinase 2 | - |
Sabellastarte spectabilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.3.3 | 2 | 3.7 | D-Arg | the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 5.8 | - |
L-Arg | 25°C | Sabellastarte spectabilis | |
2.7.3.3 | 35.4 | - |
L-Arg | the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C | Sabellastarte spectabilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.3.3 | ATP | - |
Sabellastarte spectabilis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.3.3 | Sabellastarte spectabilis | calculated from sequence | - |
4.2 |
2.7.3.3 | Sabellastarte spectabilis | calculated from sequence of cDNA | - |
6.21 |
2.7.3.3 | Sabellastarte spectabilis | calculated from sequence | - |
7.2 |