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Literature summary extracted from

  • Uda, K.; Suzuki, T.
    A novel arginine kinase with substrate specificity towards D-arginine (2007), Protein J., 26, 281-291.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.3 cloning of cDNAs into pMAL plasmid and expression in Escherichia coli as a fusion protein with MBP tag or hexameric His tag Sabellastarte spectabilis
2.7.3.3 expressed in Escherichia coli Sabellastarte spectabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.3 4.2
-
L-Arg the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C Sabellastarte spectabilis
2.7.3.3 13.9
-
D-Arg the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C Sabellastarte spectabilis
2.7.3.3 34.3
-
L-Arg 25°C Sabellastarte spectabilis
2.7.3.3 35.4
-
L-Arg 25°C Sabellastarte spectabilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.3 40000
-
2 * 40000, Superdex 75 gel filtration Sabellastarte spectabilis
2.7.3.3 42273
-
2 * 42273, calculated from sequence Sabellastarte spectabilis
2.7.3.3 42887
-
2 * 42887, calculated from sequence Sabellastarte spectabilis
2.7.3.3 42890
-
calculated from sequence of cDNA Sabellastarte spectabilis
2.7.3.3 80000
-
fusion proteins with hexameric His tag, gel filtration Sabellastarte spectabilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.3 ATP + D-Arg Sabellastarte spectabilis
-
ADP + omega-N-phospho-D-Arg
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Sabellastarte spectabilis
-
-
-
2.7.3.3 Sabellastarte spectabilis Q4AED1
-
-
2.7.3.3 Sabellastarte spectabilis Q4AED2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.3 Amberlite IR120BNA column chromatography and reverse-phase high performance liquid chromatography Sabellastarte spectabilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.3 body wall muscle
-
Sabellastarte spectabilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ATP + D-Arg 35.2% relative activity compared to L-Arg Sabellastarte spectabilis ADP + Nomega-phospho-D-Arg
-
?
2.7.3.3 ATP + D-Arg 35.2% of the activity with L-Arg Sabellastarte spectabilis ADP + Nomega-phospho-D-Arg
-
?
2.7.3.3 ATP + D-Arg
-
Sabellastarte spectabilis ADP + omega-N-phospho-D-Arg
-
?
2.7.3.3 ATP + D-Arg 7.6% of the activity with L-Arg Sabellastarte spectabilis ADP + omega-N-phospho-D-Arg
-
?
2.7.3.3 ATP + L-Arg
-
Sabellastarte spectabilis ADP + Nomega-phospho-L-Arg
-
?
2.7.3.3 ATP + L-Arg 100% activity Sabellastarte spectabilis ADP + Nomega-phospho-L-Arg
-
?
2.7.3.3 ATP + L-Arg
-
Sabellastarte spectabilis ADP + omega-N-phospho-L-Arg
-
?
2.7.3.3 ATP + lombricine 0.17% of the activity with L-Arg Sabellastarte spectabilis ADP + omega-N-phospholombricine
-
?
2.7.3.3 ATP + lombricine 3.0% of the activity with L-Arg Sabellastarte spectabilis ADP + omega-N-phospholombricine
-
?
2.7.3.3 ATP + taurocyamine 0.11% of the activity with L-Arg Sabellastarte spectabilis ADP + N-phosphotaurocyamine
-
?
2.7.3.3 ATP + taurocyamine 2.7% of the activity with L-Arg Sabellastarte spectabilis ADP + N-phosphotaurocyamine
-
?

Subunits

EC Number Subunits Comment Organism
2.7.3.3 dimer 2 * 40000, Superdex 75 gel filtration Sabellastarte spectabilis
2.7.3.3 dimer 2 * 42273, calculated from sequence Sabellastarte spectabilis
2.7.3.3 dimer 2 * 42887, calculated from sequence Sabellastarte spectabilis

Synonyms

EC Number Synonyms Comment Organism
2.7.3.3 AK1
-
Sabellastarte spectabilis
2.7.3.3 AK2
-
Sabellastarte spectabilis
2.7.3.3 arginine kinase 1
-
Sabellastarte spectabilis
2.7.3.3 arginine kinase 2
-
Sabellastarte spectabilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.3 2 3.7 D-Arg the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C Sabellastarte spectabilis
2.7.3.3 5.8
-
L-Arg 25°C Sabellastarte spectabilis
2.7.3.3 35.4
-
L-Arg the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C Sabellastarte spectabilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.3 ATP
-
Sabellastarte spectabilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.3.3 Sabellastarte spectabilis calculated from sequence
-
4.2
2.7.3.3 Sabellastarte spectabilis calculated from sequence of cDNA
-
6.21
2.7.3.3 Sabellastarte spectabilis calculated from sequence
-
7.2