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Literature summary extracted from

  • Coupe, E.E.; Smyth, M.G.; Fosberry, A.P.; Hall, R.M.; Littlechild, J.A.
    The dodecameric vanadium-dependent haloperoxidase from the marine algae Corallina officinalis: Cloning, expression, and refolding of the recombinant enzyme (2007), Protein Expr. Purif., 52, 265-272.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.18 acetonitrile sVBPO showed an increase in activity in the presence of acetonitrile, which is not observed with nVBPO or rVBPO Corallina officinalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.18 overexpressed in Escherichia coli. The enzyme is found to be predominantly in the form of inclusion bodies Corallina officinalis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.18 Corallina officinalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.18 isolation of three forms of CoVBPO: nVBPO which is isolated directly from the algal species, sVBPO which is produced in a soluble form by Escherichia coli and rVBPO which is expressed by Escherichia coli as inclusion bodies and subsequently refolded Corallina officinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.18 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Corallina officinalis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.18 CoVBPO
-
 Corallina officinalis
1.11.1.18 nVBPO enzyme which is isolated directly from the algal species Corallina officinalis
1.11.1.18 rVBPO enzyme which is expressed by Escherichia coli as inclusion bodies and subsequently refolded Corallina officinalis
1.11.1.18 sVBPO enzyme which is produced in a soluble form by Escherichia coli Corallina officinalis
1.11.1.18 vanadium-dependent bromoperoxidase
-
 Corallina officinalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.18 65
-
 sVBPO Corallina officinalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.18 60
-
 1 h, about 50% loss of activity Corallina officinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.18 7
-
 rVBPO and nVBPO Corallina officinalis
1.11.1.18 7.5
-
 sVBPO Corallina officinalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.18 5.5 10 pH 5.5: 70-90% of maximal activity depending on enzyme form, pH 10.0: about 40% of maximal activity Corallina officinalis