Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhao, C.; Kumada, Y.; Imanaka, H.; Imamura, K.; Nakanishi, K.
    Cloning, overexpression, purification, and characterization of O-acetylserine sulfhydrylase-B from Escherichia coli (2006), Protein Expr. Purif., 47, 607-613.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 overexpressed in Escherichia coli using pUC19 with a lacUV5 promoter Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 (NH4)6Mo7O24 1 mM, 61% loss of activity Escherichia coli
2.5.1.47 AgNO3 1 mM, complete loss of activity Escherichia coli
2.5.1.47 CuSO4 1 mM, 99% loss of activity Escherichia coli
2.5.1.47 FeSO4 1 mM, 99% loss of activity Escherichia coli
2.5.1.47 iodoacetamide 1 mM, 48% loss of activity Escherichia coli
2.5.1.47 PCMB 1 mM, 97% loss of activity Escherichia coli
2.5.1.47 ZnCl2 1 mM, 88% loss of activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.133
-
 sodium thiosulfate
-
 Escherichia coli
2.5.1.47 1.3
-
 O-acetyl-L-Ser
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 32600
-
 2 * 32600, SDS-PAGE Escherichia coli
2.5.1.47 64000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 728
-
 25°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + 1,2,3,4-tetrazole
-
 Escherichia coli ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + 1,2,3-benzotriazole weak activity Escherichia coli ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + 1,2,4-triazole
-
 Escherichia coli ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + 1-propanethiol
-
 Escherichia coli ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + 3-mercapto-1,2,4-triazole
-
 Escherichia coli ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + benzenethiol
-
 Escherichia coli L-cys + benzylacetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + cysteamine weak activity Escherichia coli ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + H2S
-
 Escherichia coli L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + pyrazole weak activity Escherichia coli ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + sodium azide weak activity Escherichia coli ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + sodium thiosulfate
-
 Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 32600, SDS-PAGE Escherichia coli
2.5.1.47 More OASS-B does not form a complex with Escherichia coli serine acetyltransferase (SAT, EC 2.3.1.30) Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 O-acetylserine sulfhydrylase-B
-
 Escherichia coli
2.5.1.47 OASS-B
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.47
-
 60 1 h, pH 7.5, stable Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 7.2 9
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.47 6 10 pH 6: about 55% of maximal activity, pH 10.0: about 40% of maximal activity Escherichia coli