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Literature summary extracted from

  • Yang, Y.; Xu, S.; Zhang, M.; Jin, R.; Zhang, L.; Bao, J.; Wang, H.
    Purification and characterization of a functionally active Mycobacterium tuberculosis pyrroline-5-carboxylate reductase (2006), Protein Expr. Purif., 45, 241-248.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.5.1.2 medicine pyrroline-5-carboxylate reductase is related to virulence of Mycobacterium tuberculosis Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.2 into the pET30a vector for expression in Escherichia coli BL21DE3 Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 deoxycholate
-
 Mycobacterium tuberculosis
1.5.1.2 H2O2
-
 Mycobacterium tuberculosis
1.5.1.2 methanol
-
 Mycobacterium tuberculosis
1.5.1.2 SDS
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.04
-
 1-pyrroline-5-carboxylate cofactor NADH Mycobacterium tuberculosis
1.5.1.2 0.047
-
 1-pyrroline-5-carboxylate cofactor NADPH Mycobacterium tuberculosis
1.5.1.2 0.057
-
 NADH
-
 Mycobacterium tuberculosis
1.5.1.2 0.059
-
 NADPH
-
 Mycobacterium tuberculosis
1.5.1.2 0.093
-
 NADP+
-
 Mycobacterium tuberculosis
1.5.1.2 0.12
-
 L-proline
-
 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.2 Mg2+ activity is significantly increased in the forward reaction, optimal concentration of the cation is about 50-100 microM Mycobacterium tuberculosis
1.5.1.2 Mn2+ activity is significantly increased in the forward reaction, optimal concentration of the cation is about 50-100 microM Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.2 32000
-
 after enterokinase treatment, estimated by SDS-PAGE Mycobacterium tuberculosis
1.5.1.2 365000
-
 decamer, analysis of nondegenerated protein by native gel electrophoresis Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+ Mycobacterium tuberculosis
-
 L-proline + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.2 by Ni-NTA His-binding resin affinity chromatography, the N-terminal fusion partner is cleaved by enterokinase Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.2 0.9
-
 purification step supernatant, reverse reaction Mycobacterium tuberculosis
1.5.1.2 1.7
-
 purification step supernatant, forward reaction Mycobacterium tuberculosis
1.5.1.2 30
-
 purification step Ni-NTA column, reverse reaction Mycobacterium tuberculosis
1.5.1.2 57
-
 purification step Ni-NTA column, forward reaction Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Mycobacterium tuberculosis L-proline + NAD+
-
 r
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Mycobacterium tuberculosis L-proline + NADP+
-
 r
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+ activity assay, forward reaction Mycobacterium tuberculosis L-proline + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.2 decamer 10*32000-35600, estimated by SDS-PAGE, confirmed by spectrometric analysis Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.5.1.2 P5CR
-
 Mycobacterium tuberculosis
1.5.1.2 pyrroline-5-carboxylate reductase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.2 37
-
 activity assay, forward and reverse reaction Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.5.1.2 40 50
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.2 60
-
 half life of the His-tagged protein is about 20 min Mycobacterium tuberculosis
1.5.1.2 70
-
 His-tagged protein is rapidly inactivated Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.2 0.00983
-
 1-pyrroline-5-carboxylate cofactor NADPH Mycobacterium tuberculosis
1.5.1.2 137.9
-
 NADP+
-
 Mycobacterium tuberculosis
1.5.1.2 212.8
-
 L-proline
-
 Mycobacterium tuberculosis
1.5.1.2 310.2
-
 NADH
-
 Mycobacterium tuberculosis
1.5.1.2 321.6
-
 NADPH
-
 Mycobacterium tuberculosis
1.5.1.2 387.3
-
 1-pyrroline-5-carboxylate cofactor NADH Mycobacterium tuberculosis
1.5.1.2 451.7
-
 1-pyrroline-5-carboxylate cofactor NADPH Mycobacterium tuberculosis
1.5.1.2 4000
-
 NADPH
-
 Mycobacterium tuberculosis
1.5.1.2 4170
-
 NADH
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 7.5
-
 activity assay, forward reaction Mycobacterium tuberculosis
1.5.1.2 10.3
-
 activity assay, reverse reaction Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADPH
-
 Mycobacterium tuberculosis