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Literature summary extracted from

  • Rzeznicka, K.; Walker, C.J.; Westergren, T.; Kannangara, C.G.; von Wettstein, D.; Merchant, S.; Gough, S.P.; Hansson, M.
    Xantha-l encodes a membrane subunit of the aerobic Mg-protoporphyrin IX monomethyl ester cyclase involved in chlorophyll biosynthesis (2005), Proc. Natl. Acad. Sci. USA, 102, 5886-5891.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.81 gene xantha-1, DNA and amino acid sequence determination and analysis, expression of wild-type and mutants in transgenic barley plants Hordeum vulgare

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.81 additional information construction of xantha-l35, xantha-l81, xantha-l82, viridis-k23, and viridis-k170 mutants, the mutants are all inactive in cyclase activity, phenotypes of mutant plants, overview Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.81 chloroplast
-
 Hordeum vulgare 9507
-
1.14.13.81 membrane the aerobic cyclase is composed of three gene products, a soluble protein, a membrane-bound component encoded by xantha-l, and another membrane-bound component encoded by viridis-k Hordeum vulgare 16020
-
1.14.13.81 soluble the aerobic cyclase is composed of three gene products, a soluble protein, a membrane-bound component encoded by xantha-l, and another membrane-bound component encoded by viridis-k Hordeum vulgare
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.81 Mg2+ required Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.81 magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 Hordeum vulgare the enzyme is involved in the chlorophyll biosynthesis magnesium-protochlorophyllide + NADP+ + H2O
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.81 Hordeum vulgare Q5EFU4 gene xantha-I; cv. Bonus, the aerobic cyclase is composed of three gene products, a soluble protein, a membrane-bound component encoded by xantha-l, and another membrane-bound component encoded by viridis-k
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.81 partially by chloroplast supernatant and membrane preparation of wild-type and recombinant mutant plants Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.81 leaf
-
 Hordeum vulgare
-
1.14.13.81 seedling
-
 Hordeum vulgare
-
1.14.13.81 stem
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.81 0.0000085
-
 average wild-type activity in chloroplasts Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.81 magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2
-
 Hordeum vulgare magnesium-protochlorophyllide + NADP+ + H2O
-
 ir
1.14.13.81 magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 the enzyme is involved in the chlorophyll biosynthesis Hordeum vulgare magnesium-protochlorophyllide + NADP+ + H2O
-
 ir

Subunits

EC Number Subunits Comment Organism
1.14.13.81 More the aerobic cyclase is composed of three different subunits: a soluble protein, a membrane-bound component encoded by Xantha-l, and another membrane-bound component encoded by viridis-k Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
1.14.13.81 aerobic Mg-protoporphyrin IX monomethyl ester cyclase
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.81 30
-
 assay at Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.81 7.8
-
 assay at Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.81 NADPH
-
 Hordeum vulgare