Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, T.; Zhu, P.; Cheng, K.D.; Meng, C.; Zhu, H.X.
    Molecular cloning and expression of putrescine N-methyltransferase from the hairy roots of Anisodus tanguticus (2005), Planta Med., 71, 987-989.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.53 additional information the deduced amino acid sequence of Anisodus tanguticus shows 92% identity with both Hyoscyamus niger and Atropa belladonna PMT Anisodus tanguticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.53 into pETat-32a(+) vector and expressed in Escherichia coli BL21trxB(DE3) Anisodus tanguticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.53 Ag+ 51% inhibition by 5 mM Anisodus tanguticus
2.1.1.53 Ca2+ 28% inhibition by 5 mM Anisodus tanguticus
2.1.1.53 Cr2+ 31% inhibition by 5 mM Anisodus tanguticus
2.1.1.53 Cu2+ 73% inhibition by 5 mM Anisodus tanguticus
2.1.1.53 EDTA 23% inhibition by 5 mM Anisodus tanguticus
2.1.1.53 Fe2+ 33% inhibition by 5 mM Anisodus tanguticus
2.1.1.53 Fe3+ 14% inhibition by 5 mM Anisodus tanguticus
2.1.1.53 Mg2+ 28% inhibition by 5 mM Anisodus tanguticus
2.1.1.53 Mn2+ 26% inhibition by 5 mM Anisodus tanguticus
2.1.1.53 additional information no enzyme activity without the addition of S-adenosyl-L-methionine Anisodus tanguticus
2.1.1.53 Ni2+ 71% inhibition by 5 mM Anisodus tanguticus
2.1.1.53 Zn2+ 27% inhibition by 5 mM Anisodus tanguticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.53 37000
-
 x * 37000, SDS-PAGE Anisodus tanguticus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.53 Anisodus tanguticus Q68HB1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.53 by HiTrap affinity chromatography and gel filtration Anisodus tanguticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.53 root
-
 Anisodus tanguticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Anisodus tanguticus S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.53 ? x * 37000, SDS-PAGE Anisodus tanguticus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.53 PMT
-
 Anisodus tanguticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.53 35
-
-
 Anisodus tanguticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.53 7.4
-
-
 Anisodus tanguticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.53 5 9.5
-
 Anisodus tanguticus