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Literature summary extracted from

  • Igamberdiev, A.U.; Bykova, N.V.; Hill, R.D.
    Nitric oxide scavenging by barley hemoglobin is facilitated by a monodehydroascorbate reductase-mediated ascorbate reduction of methemoglobin (2006), Planta, 223, 1033-1040.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.4 expressed in Escherichia coli Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.4 0.004
-
 NADH in the presence of hemoglobin Hordeum vulgare
1.6.5.4 0.6
-
 monodehydroascorbate in the presence of hemoglobin Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.4 cytosol
-
 Hordeum vulgare 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.4 40000
-
 SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.4 Phenyl Superose gel filtration, Blue Sepharose column chromatography and DEAE cellulose column chromatography Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.4 root
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.4 185
-
 using ascorbate free radical as substrate Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.4 NADH + monodehydroascorbate
-
 Hordeum vulgare NAD+ + ascorbate
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.5.4 monomer 1 * 40000, SDS-PAGE Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
1.6.5.4 MDHAR
-
 Hordeum vulgare