BRENDA - Enzyme Database

Extraction and partial characterization of polyphenol oxidase from banana (Musa acuminata Grande naine) roots

Wuyts, N.; De Waele, D.; Swennen, R.; Plant Physiol. Biochem. 44, 308-314 (2006)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
4-hydroxybenzoic acid
-
Musa acuminata
1.10.3.1
Citric acid
slight inhibition
Musa acuminata
1.10.3.1
dithiothreitol
complete inhibition at 0.150 mM of the root enzyme
Musa acuminata
1.10.3.1
EDTA
inhibits the root enzyme, while the pulp enzyme is only poorly inhiibited
Musa acuminata
1.10.3.1
L-ascorbic acid
complete inhibition at 0.90 mM of the root enzyme, and at 1 mM of the pulp enzyme
Musa acuminata
1.10.3.1
L-cysteine
complete inhibition at 0.973 mM of the root enzyme, and at 1 mM of the pulp enzyme
Musa acuminata
1.10.3.1
Sodium metabisulfite
complete inhibition at 0.109 mM of the root enzyme, and at 1 mM of the pulp enzyme
Musa acuminata
1.14.18.1
dithiothreitol
strong inhibition
Musa acuminata
1.14.18.1
Sodium metabisulfite
strong inhibition
Musa acuminata
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.1
additional information
-
additional information
Michaelis-Menten kinetics
Musa acuminata
1.10.3.1
0.6
-
dopamine
pH 6.5, 25°C
Musa acuminata
1.10.3.1
1.2
-
D-catechin
pH 6.5, 25°C
Musa acuminata
1.10.3.1
6.2
-
chlorogenic acid
pH 6.5, 25°C
Musa acuminata
1.10.3.1
8.3
-
4-methylcatechol
pH 6.5, 25°C
Musa acuminata
1.10.3.1
9.5
-
caffeic acid
pH 6.5, 25°C
Musa acuminata
1.10.3.1
56.3
-
L-Dopa
pH 6.5, 25°C
Musa acuminata
1.10.3.1
69
-
catechol
pH 6.5, 25°C
Musa acuminata
1.14.18.1
additional information
-
additional information
no Michaelis-Menten kinetics
Musa acuminata
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.10.3.1
D-catechin + O2
Musa acuminata
-
?
-
-
?
1.10.3.1
dopamine + O2
Musa acuminata
-
?
-
-
?
1.10.3.1
additional information
Musa acuminata
the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plant’s defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis
?
-
-
-
1.14.18.1
additional information
Musa acuminata
the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plant’s defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.10.3.1
Musa acuminata
-
-
-
1.14.18.1
Musa acuminata
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.10.3.1
optimization of enzyme extraction from roots, best using 0.2 M phosphate buffer at pH 7.0 with 5% insoluble polyvinylpyrrolidone and 0.25% Triton X-100, overview
Musa acuminata
1.14.18.1
optimization of enzyme extraction from roots, best using 0.2 M phosphate buffer at pH 7.0 with 5% insoluble polyvinylpyrrolidone and 0.25% Triton X-100, overview
Musa acuminata
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.1
fruit
pulp and peel
Musa acuminata
-
1.10.3.1
leaf
-
Musa acuminata
-
1.10.3.1
additional information
tissue distribution, overview
Musa acuminata
-
1.10.3.1
root
aerenchyma in the cortex
Musa acuminata
-
1.10.3.1
stem
-
Musa acuminata
-
1.14.18.1
fruit
pulp and peel
Musa acuminata
-
1.14.18.1
leaf
-
Musa acuminata
-
1.14.18.1
additional information
tissue distribution, overview
Musa acuminata
-
1.14.18.1
pulp
-
Musa acuminata
-
1.14.18.1
root
-
Musa acuminata
-
1.14.18.1
stem
-
Musa acuminata
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.10.3.1
additional information
-
-
Musa acuminata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
4-methylcatechol + O2
-
676649
Musa acuminata
4-methyl-1,2-benzoquinone + H2O
-
-
-
?
1.10.3.1
caffeic acid + O2
-
676649
Musa acuminata
?
-
-
-
?
1.10.3.1
catechol + 1/2 O2
low activity
676649
Musa acuminata
1,2-benzoquinone + H2O
-
-
-
?
1.10.3.1
chlorogenic acid + O2
-
676649
Musa acuminata
?
-
-
-
?
1.10.3.1
D-catechin + O2
-
676649
Musa acuminata
?
-
-
-
?
1.10.3.1
D-catechin + O2
best substrate
676649
Musa acuminata
?
-
-
-
?
1.10.3.1
dopamine + O2
-
676649
Musa acuminata
?
-
-
-
?
1.10.3.1
L-dopa + 1/2 O2
i.e. L-3,4-dihydroxyphenylalanine, low activity
676649
Musa acuminata
L-dopaquinone + H2O
-
-
-
?
1.10.3.1
additional information
the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plant’s defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis
676649
Musa acuminata
?
-
-
-
-
1.10.3.1
additional information
the enzyme catalyzes the hydroxylation of monophenols to o-diphenols, monophenolase activity EC 1.14.18.1, and the oxidation of the o-diphenols to o-quinones, diphenolase activity EC 1.10.3.1, cross-reaction analysis, overview
676649
Musa acuminata
?
-
-
-
-
1.14.18.1
4-cresol + O2
root and pulp enzyme
676649
Musa acuminata
?
-
-
-
?
1.14.18.1
additional information
the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plant’s defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis
676649
Musa acuminata
?
-
-
-
-
1.14.18.1
additional information
no activity with L-tyrosine by the root and the pulp enzyme, the enzyme catalyzes the hydroxylation of monophenols to o-diphenols, monophenolase activity EC 1.14.18.1, and the oxidation of the o-diphenols to o-quinones, diphenolase activity EC 1.10.3.1, cross-reaction analysis, overview
676649
Musa acuminata
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.1
25
-
assay at
Musa acuminata
1.14.18.1
25
-
assay at
Musa acuminata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.1
6.5
-
assay at
Musa acuminata
1.14.18.1
6.5
-
assay at
Musa acuminata
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
4-hydroxybenzoic acid
-
Musa acuminata
1.10.3.1
Citric acid
slight inhibition
Musa acuminata
1.10.3.1
dithiothreitol
complete inhibition at 0.150 mM of the root enzyme
Musa acuminata
1.10.3.1
EDTA
inhibits the root enzyme, while the pulp enzyme is only poorly inhiibited
Musa acuminata
1.10.3.1
L-ascorbic acid
complete inhibition at 0.90 mM of the root enzyme, and at 1 mM of the pulp enzyme
Musa acuminata
1.10.3.1
L-cysteine
complete inhibition at 0.973 mM of the root enzyme, and at 1 mM of the pulp enzyme
Musa acuminata
1.10.3.1
Sodium metabisulfite
complete inhibition at 0.109 mM of the root enzyme, and at 1 mM of the pulp enzyme
Musa acuminata
1.14.18.1
dithiothreitol
strong inhibition
Musa acuminata
1.14.18.1
Sodium metabisulfite
strong inhibition
Musa acuminata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.1
additional information
-
additional information
Michaelis-Menten kinetics
Musa acuminata
1.10.3.1
0.6
-
dopamine
pH 6.5, 25°C
Musa acuminata
1.10.3.1
1.2
-
D-catechin
pH 6.5, 25°C
Musa acuminata
1.10.3.1
6.2
-
chlorogenic acid
pH 6.5, 25°C
Musa acuminata
1.10.3.1
8.3
-
4-methylcatechol
pH 6.5, 25°C
Musa acuminata
1.10.3.1
9.5
-
caffeic acid
pH 6.5, 25°C
Musa acuminata
1.10.3.1
56.3
-
L-Dopa
pH 6.5, 25°C
Musa acuminata
1.10.3.1
69
-
catechol
pH 6.5, 25°C
Musa acuminata
1.14.18.1
additional information
-
additional information
no Michaelis-Menten kinetics
Musa acuminata
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.10.3.1
D-catechin + O2
Musa acuminata
-
?
-
-
?
1.10.3.1
dopamine + O2
Musa acuminata
-
?
-
-
?
1.10.3.1
additional information
Musa acuminata
the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plant’s defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis
?
-
-
-
1.14.18.1
additional information
Musa acuminata
the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plant’s defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.10.3.1
optimization of enzyme extraction from roots, best using 0.2 M phosphate buffer at pH 7.0 with 5% insoluble polyvinylpyrrolidone and 0.25% Triton X-100, overview
Musa acuminata
1.14.18.1
optimization of enzyme extraction from roots, best using 0.2 M phosphate buffer at pH 7.0 with 5% insoluble polyvinylpyrrolidone and 0.25% Triton X-100, overview
Musa acuminata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.1
fruit
pulp and peel
Musa acuminata
-
1.10.3.1
leaf
-
Musa acuminata
-
1.10.3.1
additional information
tissue distribution, overview
Musa acuminata
-
1.10.3.1
root
aerenchyma in the cortex
Musa acuminata
-
1.10.3.1
stem
-
Musa acuminata
-
1.14.18.1
fruit
pulp and peel
Musa acuminata
-
1.14.18.1
leaf
-
Musa acuminata
-
1.14.18.1
additional information
tissue distribution, overview
Musa acuminata
-
1.14.18.1
pulp
-
Musa acuminata
-
1.14.18.1
root
-
Musa acuminata
-
1.14.18.1
stem
-
Musa acuminata
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.10.3.1
additional information
-
-
Musa acuminata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
4-methylcatechol + O2
-
676649
Musa acuminata
4-methyl-1,2-benzoquinone + H2O
-
-
-
?
1.10.3.1
caffeic acid + O2
-
676649
Musa acuminata
?
-
-
-
?
1.10.3.1
catechol + 1/2 O2
low activity
676649
Musa acuminata
1,2-benzoquinone + H2O
-
-
-
?
1.10.3.1
chlorogenic acid + O2
-
676649
Musa acuminata
?
-
-
-
?
1.10.3.1
D-catechin + O2
-
676649
Musa acuminata
?
-
-
-
?
1.10.3.1
D-catechin + O2
best substrate
676649
Musa acuminata
?
-
-
-
?
1.10.3.1
dopamine + O2
-
676649
Musa acuminata
?
-
-
-
?
1.10.3.1
L-dopa + 1/2 O2
i.e. L-3,4-dihydroxyphenylalanine, low activity
676649
Musa acuminata
L-dopaquinone + H2O
-
-
-
?
1.10.3.1
additional information
the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plant’s defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis
676649
Musa acuminata
?
-
-
-
-
1.10.3.1
additional information
the enzyme catalyzes the hydroxylation of monophenols to o-diphenols, monophenolase activity EC 1.14.18.1, and the oxidation of the o-diphenols to o-quinones, diphenolase activity EC 1.10.3.1, cross-reaction analysis, overview
676649
Musa acuminata
?
-
-
-
-
1.14.18.1
4-cresol + O2
root and pulp enzyme
676649
Musa acuminata
?
-
-
-
?
1.14.18.1
additional information
the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plant’s defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis
676649
Musa acuminata
?
-
-
-
-
1.14.18.1
additional information
no activity with L-tyrosine by the root and the pulp enzyme, the enzyme catalyzes the hydroxylation of monophenols to o-diphenols, monophenolase activity EC 1.14.18.1, and the oxidation of the o-diphenols to o-quinones, diphenolase activity EC 1.10.3.1, cross-reaction analysis, overview
676649
Musa acuminata
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.1
25
-
assay at
Musa acuminata
1.14.18.1
25
-
assay at
Musa acuminata
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.1
6.5
-
assay at
Musa acuminata
1.14.18.1
6.5
-
assay at
Musa acuminata