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Literature summary extracted from

  • Baltas, M.; Lapeyre, C.; Bedos-Belval, F.; Maturano, M.; Saint-Aguet, P.; Roussel, L.; Duran, H.; Grima-Pettenati, J.
    Kinetic and inhibition studies of cinnamoyl-CoA reductase 1 from Arabidopsis thaliana (2005), Plant Physiol. Biochem., 43, 746-753.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.44 into the pGEX-4T vector for expression in Escherichia coli BL21DE3 cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.44 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(3,4-dimethoxyphenyl)ethenyl]phosphonothioate
-
 Arabidopsis thaliana
1.2.1.44 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3,5-dimethoxyphenyl)ethenyl]phosphonothioate
-
 Arabidopsis thaliana
1.2.1.44 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3-methoxyphenyl)ethenyl]phosphonothioate
-
 Arabidopsis thaliana
1.2.1.44 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxyphenyl)ethenyl]phosphonothioate
-
 Arabidopsis thaliana
1.2.1.44 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-methoxyphenyl)ethenyl]phosphonothioate
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.44 0.00042
-
 feruloyl-CoA
-
 Arabidopsis thaliana
1.2.1.44 0.00055
-
 sinapoyl-CoA
-
 Arabidopsis thaliana
1.2.1.44 0.00227
-
 p-Coumaroyl-CoA
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.44 37000
-
 determined by SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.44 cinnamaldehyde + CoA + NADP+ Arabidopsis thaliana
-
 cinnamoyl-CoA + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.44 using a glutathione-sepharose 4 Fast Flow column, purified GST-AtCCR1 is cleaved by thrombin to remove the GST tag Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 cinnamaldehyde + CoA + NADP+
-
 Arabidopsis thaliana cinnamoyl-CoA + NADPH + H+
-
 ?
1.2.1.44 feruloyl-CoA + NADPH + H+
-
 Arabidopsis thaliana coniferaldehyde + CoA + NADP+
-
 ?
1.2.1.44 p-coumaroyl-CoA + NADPH + H+
-
 Arabidopsis thaliana p-coumaraldehyde + CoA + NADP+
-
 ?
1.2.1.44 sinapoyl-CoA + NADPH + H+
-
 Arabidopsis thaliana sinapaldehyde + CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.44 AtCCR1
-
 Arabidopsis thaliana
1.2.1.44 CCR ambiguous Arabidopsis thaliana
1.2.1.44 cinnamoyl-CoA reductase
-
 Arabidopsis thaliana
1.2.1.44 cinnamoyl-CoA reductase 1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.44 30
-
 activity assay Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.44 1.63
-
 p-Coumaroyl-CoA
-
 Arabidopsis thaliana
1.2.1.44 1.67
-
 sinapoyl-CoA
-
 Arabidopsis thaliana
1.2.1.44 1.9
-
 feruloyl-CoA
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.44 6.25
-
 activity assay Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.44 NADPH
-
 Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.44 0.0044
-
 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3-methoxyphenyl)ethenyl]phosphonothioate with feruloyl-CoA as substrate, noncompetitive inhibition Arabidopsis thaliana
1.2.1.44 0.0071
-
 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3,5-dimethoxyphenyl)ethenyl]phosphonothioate with sinapoyl-CoA as substrate, noncompetitive inhibition Arabidopsis thaliana
1.2.1.44 0.031
-
 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(3,4-dimethoxyphenyl)ethenyl]phosphonothioate with feruloyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana
1.2.1.44 0.055
-
 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3,5-dimethoxyphenyl)ethenyl]phosphonothioate with feruloyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana
1.2.1.44 0.057
-
 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3-methoxyphenyl)ethenyl]phosphonothioate with sinapoyl-CoA as substrate, noncompetitive inhibition Arabidopsis thaliana
1.2.1.44 0.068
-
 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-methoxyphenyl)ethenyl]phosphonothioate with sinapoyl-CoA as substrate, noncompetitive inhibition Arabidopsis thaliana
1.2.1.44 0.156
-
 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(3,4-dimethoxyphenyl)ethenyl]phosphonothioate with sinapoyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana
1.2.1.44 0.212
-
 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxyphenyl)ethenyl]phosphonothioate with sinapoyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana
1.2.1.44 0.29
-
 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-methoxyphenyl)ethenyl]phosphonothioate with feruloyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana
1.2.1.44 0.422
-
 S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxyphenyl)ethenyl]phosphonothioate with feruloyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana