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Literature summary extracted from

  • Bogs, J.; Ebadi, A.; McDavid, D.; Robinson, S.P.
    Identification of the flavonoid hydroxylases from grapevine and their regulation during fruit development (2006), Plant Physiol., 140, 279-291.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.14.81 additional information understanding the regulation of flavonoid hydroxylases could be used to modify flavonoid composition of fruits Vitis vinifera
1.14.14.82 additional information understanding the regulation of flavonoid hydroxylases could be used to modify flavonoid composition of fruits Vitis vinifera

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.81 gene F3',5'H1, DNA and amino acid sequence determination and analysis, phylogenetic tree, developmental expression analysis, comparison of red and white cultivar enzyme expression levels, functional expression in Petunia hybrida altering the hosts' flower color and flavonoid composition Vitis vinifera
1.14.14.82 gene F3'H, DNA and amino acid sequence determination and analysis, phylogenetic tree, developmental expression analysis, comparison of red and white cultivar enzyme expression levels, functional ectopic expression in Petunia hybrida altering flower color and flavonoid composition, overview Vitis vinifera

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.81 additional information transgenic Petunia hybrida lines, overexpressing the enzyme from Vitis vinifera, produce high levels of the 3',4',5'-hydroxylated anthocyanin malvidin and very little 3',4'-hydroxylated anthocyanins, they also show a shift from kaempferol to quercetin when compared to the nontransgenic control, flower colours and phenotypes, white and red grape varieties exhibit different temporal expression of F3',5'H1 and cytochrome b5, overview Vitis vinifera

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.81 microsome
-
 Vitis vinifera
-
-
1.14.14.82 microsome
-
 Vitis vinifera
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.81 dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Vitis vinifera
-
 dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.81 additional information Vitis vinifera transcriptional regulation of the enzyme in fruits, the enzyme is expressed after flowering, when proanthocyanidins are synthesized, accumulation of hydroxylated anthocyaniins in grape berries, flavonoid biosynthetic pathways, overview ?
-
 ?
1.14.14.81 naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Vitis vinifera
-
 (2S)-5,7,3',4',5'-pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 Vitis vinifera
-
 dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 additional information Vitis vinifera flavonoid biosynthetic pathway, enzyme regulation, overview ?
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2 Vitis vinifera
-
 eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.81 Vitis vinifera Q2UYU7 cv. Shiraz, gene F3',5'H1
-
1.14.14.82 Vitis vinifera Q2UYU6 cv. Shiraz
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.81 fruit berry skin, transcriptional regulation of the enzyme, onset of expression after flowering, low expression at the onset of ripening, high expression after veraison concomitant with the accumulation of 3'- and 3',5'-hydroxylated anthocyanins, overview Vitis vinifera
-
1.14.14.81 additional information no expression in seeds Vitis vinifera
-
1.14.14.82 fruit regulation during fruit development, overview, berry skin, transcriptional regulation of the enzyme, onset of expression after flowering, low expression at the onset of ripening, high expression after veraison concomitant with the accumulation of 3'- and 3',5'-hydroxylated anthocyanins, overview Vitis vinifera
-
1.14.14.82 additional information tissue- and development-specific expression, F3'H is expressed in grapes before flowering, when 3'-hydroxylated flavonols are made, and after flowering, when proanthocyanidins are synthesized, the expression of flavonoid hydroxylases in grape is correlated to the expression of cytochrome b5, overview Vitis vinifera
-
1.14.14.82 seed F3'H is expressed consistent with the accumulation of 3'-hydroxylated proanthocyanidins in this tissue Vitis vinifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.81 dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Vitis vinifera dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.81 additional information transcriptional regulation of the enzyme in fruits, the enzyme is expressed after flowering, when proanthocyanidins are synthesized, accumulation of hydroxylated anthocyaniins in grape berries, flavonoid biosynthetic pathways, overview Vitis vinifera ?
-
 ?
1.14.14.81 naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Vitis vinifera (2S)-5,7,3',4',5'-pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Vitis vinifera dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 additional information flavonoid biosynthetic pathway, enzyme regulation, overview Vitis vinifera ?
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Vitis vinifera eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.81 F3',5'H
-
 Vitis vinifera
1.14.14.82 F3'H
-
 Vitis vinifera
1.14.14.82 flavonoid 3'-hydroxylase
-
 Vitis vinifera

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.81 cytochrome b5 expression of cytchrome b5 in grape berries is correlated to enzyme expression during fruit development phases, overview Vitis vinifera