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Literature summary extracted from
- The Arabidopsis stearoyl-acyl carrier protein-desaturase family and the contribution of leaf isoforms to oleic acid synthesis (2007), Plant Mol. Biol., 63, 257-271.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.2 | S-ACP-DES1 expressed in Escherichia coli | Arabidopsis thaliana |
| 1.14.19.2 | S-ACP-DES3 enzyme expressed in Escherichia coli | Arabidopsis thaliana |
| 1.14.19.2 | S-ACP-DES4 enzyme expressed in Escherichia coli | Arabidopsis thaliana |
| 1.14.19.2 | S-ACP-DES5 enzyme expressed in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.2 | additional information | inhibition of activity of 27% for S-ACP-DES5 in the presence of 5fold excess of unlabeled product oleoyl-[acyl-carrier protein]; inhibition of activity of 50% for S-ACP-DES4 in the presence of 5fold excess of unlabeled product oleoyl-[acyl-carrier protein]; inhibition of activity of 67% for S-ACP-DES3 in the presence of 5fold excess of unlabeled product oleoyl-[acyl-carrier protein] and 40% for S-ACP-DES3 in the presence of 5fold unlabeled product palmitoleoyl-[acyl-carrier protein], respectively; inhibition of activity of 73% for SSI2 in the presence of 5fold excess of unlabeled product oleoyl-[acyl-carrier protein] and 37% for SSI2 in the presence of 5fold unlabeled product palmitoleoyl-[acyl-carrier protein], respectively; inhibition of activity of 7% for S-ACP-DES1 in the presence of 5fold excess of unlabeled product oleoyl-[acyl-carrier protein] | Arabidopsis thaliana |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.19.2 | chloroplast stroma | predicted | Arabidopsis thaliana | 9570 | - |
| 1.14.19.2 | mitochondrion | predicted | Arabidopsis thaliana | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.2 | 44180 | - |
calculated from amino acid sequence | Arabidopsis thaliana |
| 1.14.19.2 | 45020 | - |
calculated from amino acid sequence | Arabidopsis thaliana |
| 1.14.19.2 | 45290 | - |
calculated from amino acid sequence | Arabidopsis thaliana |
| 1.14.19.2 | 45480 | - |
calculated from amino acid sequence | Arabidopsis thaliana |
| 1.14.19.2 | 45690 | - |
calculated from amino acid sequence | Arabidopsis thaliana |
| 1.14.19.2 | 45860 | - |
calculated from amino acid sequence | Arabidopsis thaliana |
| 1.14.19.2 | 46950 | - |
calculated from amino acid sequence | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + electron donor + O2 | Arabidopsis thaliana | preferred substrate for S-ACP-DES1, S-ACP-DES1 preferentially desaturates the substrate at C9 position | oleoyl-[acyl-carrier protein] + electron acceptor + H2O | - |
? |  |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + electron donor + O2 | Arabidopsis thaliana | preferred substrate for S-ACP-DES5, S-ACP-DES5 preferentially desaturates the substrate at C9 position | oleoyl-[acyl-carrier protein] + electron acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + electron donor + O2 | Arabidopsis thaliana | S-ACP-DES3 preferentially desaturates the substrate at C9 position | oleoyl-[acyl-carrier protein] + electron acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + electron donor + O2 | Arabidopsis thaliana | very low activity of S-ACP-DES4, S-ACP-DES4 preferentially desaturates the substrate at C9 position | oleoyl-[acyl-carrier protein] + electron acceptor + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.2 | Arabidopsis thaliana | O22832 | - |
- |
| 1.14.19.2 | Arabidopsis thaliana | Q84VY3 | seven isoforms | - |
| 1.14.19.2 | Arabidopsis thaliana | Q9LF04 | - |
- |
| 1.14.19.2 | Arabidopsis thaliana | Q9LF05 | - |
- |
| 1.14.19.2 | Arabidopsis thaliana | Q9M879 | - |
- |
| 1.14.19.2 | Arabidopsis thaliana | Q9M880 | - |
- |
| 1.14.19.2 | Arabidopsis thaliana | Q9M881 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.19.2 | overexpressed S-ACP-DES1 enzyme | Arabidopsis thaliana |
| 1.14.19.2 | overexpressed S-ACP-DES3 enzyme | Arabidopsis thaliana |
| 1.14.19.2 | overexpressed S-ACP-DES4 enzyme | Arabidopsis thaliana |
| 1.14.19.2 | overexpressed S-ACP-DES5 enzyme | Arabidopsis thaliana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.19.2 | flower | RT-PCR, gene-specific primers for the isoform | Arabidopsis thaliana | - |
| 1.14.19.2 | leaf | Northern blot, RT-PCR, gene-specific primers for the isoform | Arabidopsis thaliana | - |
| 1.14.19.2 | leaf | Northern blot, RT-PCR, gene-specific primers for the seven isoforms | Arabidopsis thaliana | - |
| 1.14.19.2 | root | RT-PCR, gene-specific primers for the isoform | Arabidopsis thaliana | - |
| 1.14.19.2 | silique | RT-PCR, gene-specific primers for the isoform | Arabidopsis thaliana | - |
| 1.14.19.2 | stem | RT-PCR, gene-specific primers for the isoform | Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.2 | additional information | - |
S-ACP-DES1 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein] | Arabidopsis thaliana |
| 1.14.19.2 | additional information | - |
S-ACP-DES3 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein] | Arabidopsis thaliana |
| 1.14.19.2 | additional information | - |
S-ACP-DES4 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein] | Arabidopsis thaliana |
| 1.14.19.2 | additional information | - |
S-ACP-DES5 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein] | Arabidopsis thaliana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.2 | palmitoyl-[acyl-carrier protein] + AH2 + O2 | preferred substrate for S-ACP-DES3, S-ACP-DES3 preferentially desaturates palmitoyl-[acyl-carrier protein] substrate at C9 position | Arabidopsis thaliana | palmitoleoyl-[acyl-carrier protein] + A + H2O | - |
? | |
| 1.14.19.2 | palmitoyl-[acyl-carrier protein] + AH2 + O2 | S-ACP-DES1 is poorly active on palmitoyl-[acyl-carrier protein], S-ACP-DES1 preferentially desaturates the substrate at C9 position | Arabidopsis thaliana | palmitoleoyl-[acyl-carrier protein] + A + H2O | - |
? | |
| 1.14.19.2 | palmitoyl-[acyl-carrier protein] + AH2 + O2 | S-ACP-DES4 is poorly active on palmitoyl-[acyl-carrier protein], S-ACP-DES4 preferentially desaturates the substrate at C9 position | Arabidopsis thaliana | palmitoleoyl-[acyl-carrier protein] + A + H2O | - |
? | |
| 1.14.19.2 | palmitoyl-[acyl-carrier protein] + AH2 + O2 | S-ACP-DES5 poorly active on palmitoyl-[acyl-carrier protein], S-ACP-DES5 preferentially desaturates palmitoyl-[acyl-carrier protein] substrate at C9 position | Arabidopsis thaliana | palmitoleoyl-[acyl-carrier protein] + A + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + electron donor + O2 | preferred substrate for S-ACP-DES1, S-ACP-DES1 preferentially desaturates the substrate at C9 position | Arabidopsis thaliana | oleoyl-[acyl-carrier protein] + electron acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + electron donor + O2 | preferred substrate for S-ACP-DES5, S-ACP-DES5 preferentially desaturates the substrate at C9 position | Arabidopsis thaliana | oleoyl-[acyl-carrier protein] + electron acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + electron donor + O2 | S-ACP-DES3 preferentially desaturates the substrate at C9 position | Arabidopsis thaliana | oleoyl-[acyl-carrier protein] + electron acceptor + H2O | - |
? | |
| 1.14.19.2 | stearoyl-[acyl-carrier protein] + electron donor + O2 | very low activity of S-ACP-DES4, S-ACP-DES4 preferentially desaturates the substrate at C9 position | Arabidopsis thaliana | oleoyl-[acyl-carrier protein] + electron acceptor + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.2 | S-ACP-DES1 | - |
Arabidopsis thaliana |
| 1.14.19.2 | S-ACP-DES2 | - |
Arabidopsis thaliana |
| 1.14.19.2 | S-ACP-DES3 | - |
Arabidopsis thaliana |
| 1.14.19.2 | S-ACP-DES4 | - |
Arabidopsis thaliana |
| 1.14.19.2 | S-ACP-DES5 | - |
Arabidopsis thaliana |
| 1.14.19.2 | S-ACP-DES6 | - |
Arabidopsis thaliana |
| 1.14.19.2 | SSI2/FAB2 | - |
Arabidopsis thaliana |
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