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Literature summary extracted from

  • Herl, V.; Fischer, G.; Müller-Uri, F.; Kreis, W.
    Molecular cloning and heterologous expression of progesterone 5beta-reductase from Digitalis lanata Ehrh. (2006), Phytochemistry, 67, 225-231.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.3 additional information 5beta-POR is highly conserved within the genus Digitalis and the respective genes and proteins share considerable homology to putative progesterone reductases from other plant species Digitalis lanata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.3 Sph1/Sal1 5beta-POR fragment cloned into the pQE vector and transformed into Escherichia coli strain M15(pREP4) Digitalis lanata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.3 0.008
-
 NADPH
-
 Digitalis lanata
1.3.1.3 0.12
-
 progesterone
-
 Digitalis lanata
1.3.1.3 0.228
-
 4-androstene-3,17-dione
-
 Digitalis lanata
1.3.1.3 0.291
-
 cortisol
-
 Digitalis lanata
1.3.1.3 1.597
-
 cortexone
-
 Digitalis lanata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.3 40000
-
 x * 40000, SDS–PAGE Digitalis lanata

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Digitalis lanata Q6PQJ9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.3 by Ni-NTA affinity chromatography Digitalis lanata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.3 leaf
-
 Digitalis lanata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.3 4-androstene-3,17-dione + NADPH
-
 Digitalis lanata 5beta-androstan-3,17-dione + NADP+
-
 ?
1.3.1.3 cortexone + NADPH
-
 Digitalis lanata 21-trihydroxy-5beta-pregnane-3,20-dione + NADP+
-
 ?
1.3.1.3 cortisol + NADPH
-
 Digitalis lanata 11beta,17,21-trihydroxy-5beta-pregnane-3,20-dione + NADP+
-
 ?
1.3.1.3 additional information pregnenolone, 21-OH-pregenenolone and isoprogesterone are not accepted by recombinant 5beta-POR Digitalis lanata ?
-
 ?
1.3.1.3 progesterone + NADPH + H+ 5alpha-pregnane-3,20-dione, the 5alpha-isomer of the pregnane-3,20-dione is not formed Digitalis lanata 5beta-pregnane-3,20-dione + NADP+
-
 ir
1.3.1.3 testosterone + NADPH + H+
-
 Digitalis lanata (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.3 ? x * 40000, SDS–PAGE Digitalis lanata

Synonyms

EC Number Synonyms Comment Organism
1.3.1.3 5beta-POR
-
 Digitalis lanata
1.3.1.3 progesterone 5beta-reductase
-
 Digitalis lanata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.3 40
-
 with progesterone as substrate Digitalis lanata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.3 7.8
-
 with progesterone as substrate Digitalis lanata

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.3 NADPH cannot be replaced by NADH Digitalis lanata