Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamamoto, H.; Senda, M.; Inoue, K.
    Flavanone 8-dimethylallyltransferase in Sophora flavescens cell suspension cultures (2000), Phytochemistry, 54, 649-655.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.70 0.036
-
 (2S)-naringenin pH 9.0, 25°C, microsome Sophora flavescens
2.5.1.70 0.12
-
 3,3-dimethylallyl diphosphate pH 9.0, 25°C, microsome Sophora flavescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.70 microsome
-
 Sophora flavescens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.70 Ca2+ 6% of the activity with Mg2+ Sophora flavescens
2.5.1.70 Co2+ 5% of the activity with Mg2+ Sophora flavescens
2.5.1.70 Cu2+ 1% of the activity with Mg2+ Sophora flavescens
2.5.1.70 Mg2+ required, best divalent cation, optimal at 10 mM Sophora flavescens
2.5.1.70 Mn2+ 32% of the activity with Mg2+ Sophora flavescens
2.5.1.70 Zn2+ 2% of the activity with Mg2+ Sophora flavescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.70 3,3-dimethylallyl diphosphate + (2S)-naringenin Sophora flavescens two possible pathways leading to sophoraflavanone G, overview diphosphate + sophoraflavanone B
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.70 Sophora flavescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.70 native enzyme partially by microsome preparation Sophora flavescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.70 3,3-dimethylallyl diphosphate + (2S)-liquiritigenin i.e. 4',7-dihydroxyflavanone Sophora flavescens diphosphate + isobavachin
-
 ?
2.5.1.70 3,3-dimethylallyl diphosphate + (2S)-naringenin two possible pathways leading to sophoraflavanone G, overview Sophora flavescens diphosphate + sophoraflavanone B
-
 ?
2.5.1.70 3,3-dimethylallyl diphosphate + (2S)-naringenin best substrate, the enzyme is enantiospecific for (2S)-naringenin, i.e. (S)-4',5,7-trihydroxy-flavanone, and utilizes 3,3-dimethylallyl diphosphate as sole prenyl donor Sophora flavescens diphosphate + sophoraflavanone B
-
 ?
2.5.1.70 3,3-dimethylallyl diphosphate + 2'-hydroxynaringenin with 2'-hydroxynaringenin, i.e. (S)-2',4',5,7-tetrahydroxy-flavanone, the enzyme shows 3% of the activity with naringenin Sophora flavescens diphosphate + leachianone G
-
 ?
2.5.1.70 3,3-dimethylallyl diphosphate + hesperetin i.e. 5,7,3'-trihydroxy-4'-methoxyflavanone Sophora flavescens diphosphate + (2S)-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)-8-(3-methylbut-2-en-1-yl)-2,3-dihydro-4H-chromen-4-one
-
 ?
2.5.1.70 3,3-dimethylallyl diphosphate + taxifolin i.e. 3,5,7,3',4'-pentahydroxyflavanone Sophora flavescens diphosphate + (2R,3R)-2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-8-(3-methylbut-2-en-1-yl)-2,3-dihydro-4H-chromen-4-one
-
 ?
2.5.1.70 additional information substrate specificity, formation of 8-dimethylallyl-, 8-lavandulyl- and 6-dimethylallylflavanones, overview Sophora flavescens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.70 flavanone 8-dimethylallyltransferase
-
 Sophora flavescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.70 25
-
 assay at Sophora flavescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.70 9 10
-
 Sophora flavescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.70 7.5 10
-
 Sophora flavescens