EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.22 | overexpression of wild-type and mutants in Escherichia coli | Acinetobacter sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.22 | F432I | random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme | Acinetobacter sp. |
1.14.13.22 | F432S | random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme | Acinetobacter sp. |
1.14.13.22 | F432Y/K500R | random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme | Acinetobacter sp. |
1.14.13.22 | K78E/F432S | random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme | Acinetobacter sp. |
1.14.13.22 | L426P/A541V | random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme | Acinetobacter sp. |
1.14.13.22 | additional information | screening of random mutants of the enzyme against a library of structurally diverse ketones for modifications in the substrate acceptance profile and stereopreference of the enzymatic Baeyer-Villiger biooxidation, improved and/or divergent stereoselectivities are observed for several substrates, overview | Acinetobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.22 | 4-hydroxy-cyclohexanone + NADPH + O2 | Acinetobacter sp. | - |
4-hydroxy-hexano-6-lactone + NADP+ + H2O | - |
? | |
1.14.13.22 | 4-hydroxy-cyclohexanone + NADPH + O2 | Acinetobacter sp. NCIMB 9871 | - |
4-hydroxy-hexano-6-lactone + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclohexanone + NADPH + O2 | Acinetobacter sp. | - |
hexano-6-lactone + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclohexanone + NADPH + O2 | Acinetobacter sp. NCIMB 9871 | - |
hexano-6-lactone + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.22 | Acinetobacter sp. | - |
- |
- |
1.14.13.22 | Acinetobacter sp. NCIMB 9871 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.22 | 4-hydroxy-cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 4-hydroxy-hexano-6-lactone + NADP+ + H2O | - |
? | |
1.14.13.22 | 4-hydroxy-cyclohexanone + NADPH + O2 | an enantioselective Baeyer-Villiger oxidation | Acinetobacter sp. | 4-hydroxy-hexano-6-lactone + NADP+ + H2O | - |
? | |
1.14.13.22 | 4-hydroxy-cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. NCIMB 9871 | 4-hydroxy-hexano-6-lactone + NADP+ + H2O | - |
? | |
1.14.13.22 | 4-hydroxy-cyclohexanone + NADPH + O2 | an enantioselective Baeyer-Villiger oxidation | Acinetobacter sp. NCIMB 9871 | 4-hydroxy-hexano-6-lactone + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | hexano-6-lactone + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. NCIMB 9871 | hexano-6-lactone + NADP+ + H2O | - |
? | |
1.14.13.22 | additional information | substrate specificity and enantioselectivity of wild-type and mutant enzymes, overview | Acinetobacter sp. | ? | - |
? | |
1.14.13.22 | additional information | substrate specificity and enantioselectivity of wild-type and mutant enzymes, overview | Acinetobacter sp. NCIMB 9871 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.22 | CHMO | - |
Acinetobacter sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.22 | NADPH | - |
Acinetobacter sp. |