BRENDA - Enzyme Database

Enantioselectivity of human AMP, dTMP and UMP-CMP kinases

Alexandre, J.A.; Roy, B.; Topalis, D.; Pochet, S.; Perigaud, C.; Deville-Bonne, D.; Nucleic Acids Res. 35, 4895-4904 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.14
overexpression in Escherichia coli
Homo sapiens
2.7.4.25
overexpression in Escherichia coli
Escherichia coli
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.7.4.25
of the R188M mutant alone or with dCMP
Escherichia coli
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.4.25
D132A
site directed mutagenesis
Escherichia coli
2.7.4.25
D132H
site directed mutagenesis
Escherichia coli
2.7.4.25
D132N
site directed mutagenesis
Escherichia coli
2.7.4.25
D132S
site directed mutagenesis
Escherichia coli
2.7.4.25
R110M
site directed mutagenesis
Escherichia coli
2.7.4.25
R188M
site directed mutagenesis
Escherichia coli
2.7.4.25
S36A
site directed mutagenesis
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
L-CMP
excess of L-CMP
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.9
0.02
-
D-dTMP
-
Homo sapiens
2.7.4.9
0.38
-
L-dTMP
-
Homo sapiens
2.7.4.14
0.02
-
D-CMP
-
Homo sapiens
2.7.4.14
0.05
-
D-UMP
-
Homo sapiens
2.7.4.14
0.15
-
L-3TCMP
-
Homo sapiens
2.7.4.14
0.36
-
beta-D-2'-azido-2'-dCMP
-
Homo sapiens
2.7.4.14
0.7
-
L-dUMP
L-enantiomers have lower reaction rates
Homo sapiens
2.7.4.14
0.73
-
L-dCMP
L-enantiomers have lower reaction rates
Homo sapiens
2.7.4.14
0.75
-
L-CMP
-
Homo sapiens
2.7.4.14
0.9
-
beta-D-2'-azido-2'-dUMP
-
Homo sapiens
2.7.4.14
1
-
D-dCMP
-
Homo sapiens
2.7.4.14
1.2
-
beta-L-2'-azido-2'-dCMP
-
Homo sapiens
2.7.4.14
1.3
-
beta-L-2'-azido-2'-dUMP
-
Homo sapiens
2.7.4.14
1.3
-
D-dUMP
-
Homo sapiens
2.7.4.25
0.035
-
CMP
wild type
Escherichia coli
2.7.4.25
0.038
-
CMP
D132S mutant
Escherichia coli
2.7.4.25
0.055
-
dCMP
D132H mutant
Escherichia coli
2.7.4.25
0.08
-
dCMP
D132N mutant
Escherichia coli
2.7.4.25
0.09
-
dCMP
D132S mutant
Escherichia coli
2.7.4.25
0.094
-
dCMP
wild type
Escherichia coli
2.7.4.25
0.77
-
dCMP
R110M mutant
Escherichia coli
2.7.4.25
0.93
-
UMP
wild type
Escherichia coli
2.7.4.25
1
-
CMP
R110M mutant
Escherichia coli
2.7.4.25
1.3
-
CMP
D132H mutant
Escherichia coli
2.7.4.25
1.8
-
dCMP
D132A mutant
Escherichia coli
2.7.4.25
1.9
-
UMP
S36A mutant
Escherichia coli
2.7.4.25
2.5
-
CMP
S36A mutant
Escherichia coli
2.7.4.25
2.6
-
CMP
D132N mutant
Escherichia coli
2.7.4.25
2.9
-
CMP
D132A mutant
Escherichia coli
2.7.4.25
3.5
-
dCMP
S36A mutant
Escherichia coli
2.7.4.25
3.9
-
UMP
D132H mutant
Escherichia coli
2.7.4.25
4.5
-
UMP
D132N mutant
Escherichia coli
2.7.4.25
7.3
-
dCMP
R110M mutant
Escherichia coli
2.7.4.25
7.9
-
UMP
D132A mutant
Escherichia coli
2.7.4.25
8
-
UMP
D132S mutant
Escherichia coli
2.7.4.25
11.3
-
UMP
R110M mutant
Escherichia coli
2.7.4.25
20.2
-
CMP
R110M mutant
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.9
L-dTMP + ATP
Homo sapiens
-
L-dTDP + ADP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.9
Homo sapiens
P23919
-
-
2.7.4.14
Homo sapiens
P30085
-
-
2.7.4.25
Escherichia coli
P0A6I0
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.14
of the recombinant proteins
Homo sapiens
2.7.4.25
of the recombinant proteins
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.9
D-dTMP + ATP
-
676227
Homo sapiens
D-dTDP + ADP
-
-
-
?
2.7.4.9
L-dTMP + ATP
-
676227
Homo sapiens
L-dTDP + ADP
-
-
-
?
2.7.4.14
ATP + beta-D-2'-azido-2'-dCMP
-
676227
Homo sapiens
ADP + beta-D-2'-azido-2'-dCDP
-
-
-
?
2.7.4.14
ATP + beta-D-2'-azido-2'-dUMP
-
676227
Homo sapiens
ADP + beta-D-2'-azido-2'-dUDP
-
-
-
?
2.7.4.14
ATP + beta-L-2'-azido-2'-dCMP
-
676227
Homo sapiens
ADP + beta-L-2'-azido-2'-dCDP
-
-
-
?
2.7.4.14
ATP + beta-L-2'-azido-2'-dUMP
-
676227
Homo sapiens
ADP + beta-L-2'-azido-2'-dUDP
-
-
-
?
2.7.4.14
ATP + D-CMP
-
676227
Homo sapiens
ADP + D-CDP
-
-
-
?
2.7.4.14
ATP + D-dCMP
-
676227
Homo sapiens
ADP + D-dCDP
-
-
-
?
2.7.4.14
ATP + D-dUMP
-
676227
Homo sapiens
ADP + D-dUDP
-
-
-
?
2.7.4.14
ATP + D-UMP
-
676227
Homo sapiens
ADP + D-UDP
-
-
-
?
2.7.4.14
ATP + L-3TCMP
-
676227
Homo sapiens
ADP + L-3TCMP
-
-
-
?
2.7.4.14
ATP + L-CMP
poor substrate
676227
Homo sapiens
ADP + L-CDP
-
-
-
?
2.7.4.14
ATP + L-dCMP
slower phosphorylation than the D-enantiomers
676227
Homo sapiens
ADP + L-dCDP
-
-
-
?
2.7.4.14
ATP + L-dUMP
slower phosphorylation than the D-enantiomers
676227
Homo sapiens
ADP + L-dUDP
-
-
-
?
2.7.4.25
ATP + CMP
-
676227
Escherichia coli
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
-
676227
Escherichia coli
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + UMP
-
676227
Escherichia coli
ADP + UDP
-
-
-
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.4.25
43
-
mutant D132A
Escherichia coli
2.7.4.25
45
-
mutant R110M
Escherichia coli
2.7.4.25
47
-
mutant D132N
Escherichia coli
2.7.4.25
48
-
mutant D132S, mutant D132H
Escherichia coli
2.7.4.25
51
-
mutant R188M
Escherichia coli
2.7.4.25
52
-
wild type, mutant S36A
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
overexpression in Escherichia coli
Homo sapiens
2.7.4.25
overexpression in Escherichia coli
Escherichia coli
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.4.25
of the R188M mutant alone or with dCMP
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.4.25
D132A
site directed mutagenesis
Escherichia coli
2.7.4.25
D132H
site directed mutagenesis
Escherichia coli
2.7.4.25
D132N
site directed mutagenesis
Escherichia coli
2.7.4.25
D132S
site directed mutagenesis
Escherichia coli
2.7.4.25
R110M
site directed mutagenesis
Escherichia coli
2.7.4.25
R188M
site directed mutagenesis
Escherichia coli
2.7.4.25
S36A
site directed mutagenesis
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
L-CMP
excess of L-CMP
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.9
0.02
-
D-dTMP
-
Homo sapiens
2.7.4.9
0.38
-
L-dTMP
-
Homo sapiens
2.7.4.14
0.02
-
D-CMP
-
Homo sapiens
2.7.4.14
0.05
-
D-UMP
-
Homo sapiens
2.7.4.14
0.15
-
L-3TCMP
-
Homo sapiens
2.7.4.14
0.36
-
beta-D-2'-azido-2'-dCMP
-
Homo sapiens
2.7.4.14
0.7
-
L-dUMP
L-enantiomers have lower reaction rates
Homo sapiens
2.7.4.14
0.73
-
L-dCMP
L-enantiomers have lower reaction rates
Homo sapiens
2.7.4.14
0.75
-
L-CMP
-
Homo sapiens
2.7.4.14
0.9
-
beta-D-2'-azido-2'-dUMP
-
Homo sapiens
2.7.4.14
1
-
D-dCMP
-
Homo sapiens
2.7.4.14
1.2
-
beta-L-2'-azido-2'-dCMP
-
Homo sapiens
2.7.4.14
1.3
-
beta-L-2'-azido-2'-dUMP
-
Homo sapiens
2.7.4.14
1.3
-
D-dUMP
-
Homo sapiens
2.7.4.25
0.035
-
CMP
wild type
Escherichia coli
2.7.4.25
0.038
-
CMP
D132S mutant
Escherichia coli
2.7.4.25
0.055
-
dCMP
D132H mutant
Escherichia coli
2.7.4.25
0.08
-
dCMP
D132N mutant
Escherichia coli
2.7.4.25
0.09
-
dCMP
D132S mutant
Escherichia coli
2.7.4.25
0.094
-
dCMP
wild type
Escherichia coli
2.7.4.25
0.77
-
dCMP
R110M mutant
Escherichia coli
2.7.4.25
0.93
-
UMP
wild type
Escherichia coli
2.7.4.25
1
-
CMP
R110M mutant
Escherichia coli
2.7.4.25
1.3
-
CMP
D132H mutant
Escherichia coli
2.7.4.25
1.8
-
dCMP
D132A mutant
Escherichia coli
2.7.4.25
1.9
-
UMP
S36A mutant
Escherichia coli
2.7.4.25
2.5
-
CMP
S36A mutant
Escherichia coli
2.7.4.25
2.6
-
CMP
D132N mutant
Escherichia coli
2.7.4.25
2.9
-
CMP
D132A mutant
Escherichia coli
2.7.4.25
3.5
-
dCMP
S36A mutant
Escherichia coli
2.7.4.25
3.9
-
UMP
D132H mutant
Escherichia coli
2.7.4.25
4.5
-
UMP
D132N mutant
Escherichia coli
2.7.4.25
7.3
-
dCMP
R110M mutant
Escherichia coli
2.7.4.25
7.9
-
UMP
D132A mutant
Escherichia coli
2.7.4.25
8
-
UMP
D132S mutant
Escherichia coli
2.7.4.25
11.3
-
UMP
R110M mutant
Escherichia coli
2.7.4.25
20.2
-
CMP
R110M mutant
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.9
L-dTMP + ATP
Homo sapiens
-
L-dTDP + ADP
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
of the recombinant proteins
Homo sapiens
2.7.4.25
of the recombinant proteins
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.9
D-dTMP + ATP
-
676227
Homo sapiens
D-dTDP + ADP
-
-
-
?
2.7.4.9
L-dTMP + ATP
-
676227
Homo sapiens
L-dTDP + ADP
-
-
-
?
2.7.4.14
ATP + beta-D-2'-azido-2'-dCMP
-
676227
Homo sapiens
ADP + beta-D-2'-azido-2'-dCDP
-
-
-
?
2.7.4.14
ATP + beta-D-2'-azido-2'-dUMP
-
676227
Homo sapiens
ADP + beta-D-2'-azido-2'-dUDP
-
-
-
?
2.7.4.14
ATP + beta-L-2'-azido-2'-dCMP
-
676227
Homo sapiens
ADP + beta-L-2'-azido-2'-dCDP
-
-
-
?
2.7.4.14
ATP + beta-L-2'-azido-2'-dUMP
-
676227
Homo sapiens
ADP + beta-L-2'-azido-2'-dUDP
-
-
-
?
2.7.4.14
ATP + D-CMP
-
676227
Homo sapiens
ADP + D-CDP
-
-
-
?
2.7.4.14
ATP + D-dCMP
-
676227
Homo sapiens
ADP + D-dCDP
-
-
-
?
2.7.4.14
ATP + D-dUMP
-
676227
Homo sapiens
ADP + D-dUDP
-
-
-
?
2.7.4.14
ATP + D-UMP
-
676227
Homo sapiens
ADP + D-UDP
-
-
-
?
2.7.4.14
ATP + L-3TCMP
-
676227
Homo sapiens
ADP + L-3TCMP
-
-
-
?
2.7.4.14
ATP + L-CMP
poor substrate
676227
Homo sapiens
ADP + L-CDP
-
-
-
?
2.7.4.14
ATP + L-dCMP
slower phosphorylation than the D-enantiomers
676227
Homo sapiens
ADP + L-dCDP
-
-
-
?
2.7.4.14
ATP + L-dUMP
slower phosphorylation than the D-enantiomers
676227
Homo sapiens
ADP + L-dUDP
-
-
-
?
2.7.4.25
ATP + CMP
-
676227
Escherichia coli
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
-
676227
Escherichia coli
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + UMP
-
676227
Escherichia coli
ADP + UDP
-
-
-
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.4.25
43
-
mutant D132A
Escherichia coli
2.7.4.25
45
-
mutant R110M
Escherichia coli
2.7.4.25
47
-
mutant D132N
Escherichia coli
2.7.4.25
48
-
mutant D132S, mutant D132H
Escherichia coli
2.7.4.25
51
-
mutant R188M
Escherichia coli
2.7.4.25
52
-
wild type, mutant S36A
Escherichia coli