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Literature summary extracted from

  • Dombrecht, B.; Heusdens, C.; Beullens, S.; Verreth, C.; Mulkers, E.; Proost, P.; Vanderleyden, J.; Michiels, J.
    Defence of Rhizobium etli bacteroids against oxidative stress involves a complexly regulated atypical 2-Cys peroxiredoxin (2005), Mol. Microbiol., 55, 1207-1221.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expression in Escherichia coli Rhizobium etli

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.24 C156S no peroxidase activity Rhizobium etli
1.11.1.24 C56S no peroxidase activity Rhizobium etli
1.11.1.24 C81S although the PrxS C81S mutant protein can be overexpressed and purified under denaturing conditions, it is not possible to obtain any active C81S PrxS. the C81S mutant is prone to inclusion body formation Rhizobium etli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.24 0.062
-
 H2O2
-
 Rhizobium etli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 20080
-
 x * 20080, calculated from sequence Rhizobium etli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.24 additional information Rhizobium etli constitutive expression of prxS confers enhanced survival and growth to Rhizobium etli in presence of H2O2. Defence of Rhizobium etli bacteroids against oxidative stress involves a complexly regulated atypical 2-Cys peroxiredoxin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Rhizobium etli O69777
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24
-
 Rhizobium etli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.24 bacteroid the enzyme is strongly expressed under microaerobic conditions and during the sambiotic interaction with Phaseolus vulgaris Rhizobium etli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 H2O2 + dithiothreitol
-
 Rhizobium etli H2O + oxidized dithiothreitol
-
 ?
1.11.1.24 H2O2 + reduced thioredoxin
-
 Rhizobium etli H2O + oxidized thioredoxin
-
 ?
1.11.1.24 additional information constitutive expression of prxS confers enhanced survival and growth to Rhizobium etli in presence of H2O2. Defence of Rhizobium etli bacteroids against oxidative stress involves a complexly regulated atypical 2-Cys peroxiredoxin Rhizobium etli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 ? x * 20080, calculated from sequence Rhizobium etli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.24 Rhizobium etli calculated from sequence
-
 5.3