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Literature summary extracted from

  • Pantazaki, A.A.; Ioannou, A.K.; Kyriakidis, D.A.
    A thermostable beta-ketothiolase of polyhydroxyalkanoates (PHAs) in Thermus thermophilus: purification and biochemical properties (2005), Mol. Cell. Biochem., 269, 27-36.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.16 ATP activates Thermus thermophilus
2.3.1.16 NADH activates Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.16 acetoacetyl-CoA substrate inhibition at high concentrations Thermus thermophilus
2.3.1.16 NEM inhibition at 1 mM in absence or presence of 0.5 mM acetyl-CoA Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.16 additional information
-
 additional information kinetics Thermus thermophilus
2.3.1.16 0.011
-
 acetyl-CoA pH 8.0, 65°C Thermus thermophilus
2.3.1.16 0.025
-
 CoA pH 8.0, 65°C Thermus thermophilus
2.3.1.16 0.025
-
 acetoacetyl-CoA pH 8.0, 65°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.16 Ca2+ activates Thermus thermophilus
2.3.1.16 Mg2+ activates Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.16 45500
-
 4 * 45500, SDS-PAGE Thermus thermophilus
2.3.1.16 182000
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.16 acetyl-CoA + H2O Thermus thermophilus
-
 acetate + CoA
-
 r
2.3.1.16 acetyl-CoA + H2O Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 acetate + CoA
-
 r
2.3.1.16 additional information Thermus thermophilus first enzyme of the most common biosynthetic pathway for polyhydroxyalkanoates, overview ?
-
 ?
2.3.1.16 additional information Thermus thermophilus HB8 / ATCC 27634 / DSM 579 first enzyme of the most common biosynthetic pathway for polyhydroxyalkanoates, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.16 Thermus thermophilus
-
-
-
2.3.1.16 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.16 native enzyme 386.4fold to homogeneity by anion exchange, hydrophobic interaction, and hydroxylapatite chromatography, and gel filtration Thermus thermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.16 acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA ping-pong mechanism, a cysteine residue in involved in the active site Thermus thermophilus
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.16 in situ renaturation of the enzyme from the SDS-polyacrylamide gel, after solubilization, SDS removal and denaturation with 6 M guanidine hydrochloride, overview Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.16 1082
-
 purified enzyme Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.16 acetyl-CoA + H2O
-
 Thermus thermophilus acetate + CoA
-
 r
2.3.1.16 acetyl-CoA + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 acetate + CoA
-
 r
2.3.1.16 coenzyme A + acetoacetyl-CoA
-
 Thermus thermophilus 2 acetyl-CoA
-
 ?
2.3.1.16 coenzyme A + acetoacetyl-CoA
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 2 acetyl-CoA
-
 ?
2.3.1.16 additional information first enzyme of the most common biosynthetic pathway for polyhydroxyalkanoates, overview Thermus thermophilus ?
-
 ?
2.3.1.16 additional information first enzyme of the most common biosynthetic pathway for polyhydroxyalkanoates, overview Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.16 tetramer 4 * 45500, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.16 beta-ketothiolase
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.16 65
-
 for both reaction directions Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.16 65
-
 15 min, purified enzyme, completely stable Thermus thermophilus
2.3.1.16 100
-
 15 min, purified enzyme, loss of 23% of activity Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.16 8
-
 for both reaction directions Thermus thermophilus