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Literature summary extracted from

  • Bhaskara, V.; Dupre, A.; Lengsfeld, B.; Hopkins, B.B.; Chan, A.; Lee, J.H.; Zhang, X.; Gautier, J.; Zakian, V.; Paull, T.T.
    Rad50 adenylate kinase activity regulates DNA tethering by Mre11/Rad50 complexes (2007), Mol. Cell, 25, 647-661.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.3 S1202R mutation of RAD50 signature motif, 10-50% of wild-type activity in formation of ADP, formation of ATP is not affected Homo sapiens
2.7.4.3 S1205R mutation of RAD50 signature motif, 5-10% of wild-type activity in formation of ADP, formation of ATP is not affected. Complete loss of spore viability in the wild-type/S1205R heterozygote Saccharomyces cerevisiae
2.7.4.3 S1205R mutation in Rad50 protein, mutant protein is expressed in yeast and forms a complete complex with Mre11. Mutant does not support spore viability. telomeres are as severely shortened as in a rad50 deletion strain Saccharomyces cerevisiae
2.7.4.3 S793R mutation in Rad50 singature motif. Mutant shows lower levels of both ATPase and adenylate kinase activity relative to the wild-type enzyme, consistent with an overall deficiency in ATP binding Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.3 adenosine 5'-pentaphosphate inhibits the RAD50 phosphoryl transfer reaction but not ATP hydrolysis Homo sapiens
2.7.4.3 adenosine 5'-pentaphosphate inhibits the RAD50 phosphoryl transfer reaction but not ATP hydrolysis Saccharomyces cerevisiae
2.7.4.3 P1,P5-di(adenosine-5')pentaphosphate inhibitory to Rad50 phosphoryl transfer reaction but not to ATP hydrolysis Homo sapiens
2.7.4.3 P1,P5-di(adenosine-5')pentaphosphate completely abolishes adenylate kinase activity but does not affect ATPase activity Pyrococcus furiosus
2.7.4.3 P1,P5-di(adenosine-5')pentaphosphate inhibitory to Rad50 phosphoryl transfer reaction but not to ATP hydrolysis. Inhibitor blocks DNA tethering in vitro and in cell-free extracts Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.3 additional information Xenopus laevis adenylate kinase activity Is required for Mre11/Rad50-mediated DNA tethering ?
-
 ?
2.7.4.3 additional information Homo sapiens adenylate kinase activity of the Mre11/Rad50 complex, which is part of a DNA repair complex, promotes DNA-DNA associations ?
-
 ?
2.7.4.3 additional information Saccharomyces cerevisiae Rad50 adenylate kinase activity is required for DNA tethering ?
-
 ?
2.7.4.3 additional information Pyrococcus furiosus Rad50 adenylate kinase activity is required for DNA tethering ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Homo sapiens
-
 DNA repair complex components Mre11 and Rad50 show adenylate kinase activity when in complex Mre11/Rad50
-
2.7.4.3 Homo sapiens
-
 Mre11/Rad50 complex, part of a DNA repair complex
-
2.7.4.3 Pyrococcus furiosus
-
 Mre11/Rad50 complex, part of a DNA repair complex
-
2.7.4.3 Saccharomyces cerevisiae
-
 DNA repair complex components Mre11 and Rad50 show adenylate kinase activity when in complex Mre11/Rad50
-
2.7.4.3 Saccharomyces cerevisiae
-
 Mre11/Rad50 complex, part of a DNA repair complex
-
2.7.4.3 Xenopus laevis
-
 DNA repair complex components Mre11 and Rad50 show adenylate kinase activity when in complex Mre11/Rad50
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ADP
-
 Homo sapiens ATP + AMP
-
 r
2.7.4.3 ADP
-
 Saccharomyces cerevisiae ATP + AMP
-
 ?
2.7.4.3 ADP
-
 Xenopus laevis ATP + AMP
-
 r
2.7.4.3 ADP
-
 Homo sapiens AMP + ATP
-
 r
2.7.4.3 ADP
-
 Saccharomyces cerevisiae AMP + ATP
-
 r
2.7.4.3 ADP
-
 Pyrococcus furiosus AMP + ATP
-
 r
2.7.4.3 ATP + AMP
-
 Homo sapiens 2 ADP
-
 r
2.7.4.3 ATP + AMP
-
 Saccharomyces cerevisiae 2 ADP
-
 ?
2.7.4.3 ATP + AMP
-
 Xenopus laevis 2 ADP
-
 r
2.7.4.3 additional information adenylate kinase activity Is required for Mre11/Rad50-mediated DNA tethering Xenopus laevis ?
-
 ?
2.7.4.3 additional information adenylate kinase activity of the Mre11/Rad50 complex, which is part of a DNA repair complex, promotes DNA-DNA associations Homo sapiens ?
-
 ?
2.7.4.3 additional information Rad50 adenylate kinase activity is required for DNA tethering Saccharomyces cerevisiae ?
-
 ?
2.7.4.3 additional information Rad50 adenylate kinase activity is required for DNA tethering Pyrococcus furiosus ?
-
 ?