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Literature summary extracted from

  • Zhang, C.; Meng, Q.; Gai, J.; Yu, D.
    Cloning and functional characterization of an O-acetylserine(thiol)lyase-encoding gene in wild soybean (Glycine soja) (2008), Mol. Biol. Rep., 35, 527-534.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 expression in Escherichia coli Glycine max
2.7.7.48
-
 Gossypium hirsutum

General Stability

EC Number General Stability Organism
2.7.9.3 labile in 0.12% sucrose monolaurate, 1% sodium cholate, and 0.2 M sodium thiocyanate, slightly stable in 2% MEGA-9, 1% sodium deoxycholate, 1% nonidet P-40 and 1% polyoxyethylene 9 lauryl ether Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.9.3 cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.3 33000
-
 SDS-PAGE Bos taurus
2.7.9.3 35590
-
 calculated from amino acid sequence Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn Gossypium hirsutum inducible enzyme diphosphate + RNAn+1
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.9.3 Triton X-100 slightly stable in 1% (v/v) Triton X-100 Bos taurus
2.7.9.3 urea labile in 2 M urea Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Glycine max
-
-
-
2.7.7.48 Gossypium hirsutum
-
-
-
2.7.9.3 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Glycine max
2.7.9.3 DEAE-cellulose column chromatography, Sephacryl S-300 gel filtration, Heparin Toyopearl column chromatography, and hydroxylapatite column chromatography Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.9.3 ATP + selenide + H2O = AMP + selenophosphate + phosphate bovine active SPS2 does not contain selenocysteine Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 root
-
 Glycine max
-
2.5.1.47 seed
-
 Glycine max
-
2.7.9.3 liver
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
2.7.9.3 -80°C, in 0.2 M Hepes buffer at pH 6.8, less than one year, stable Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Gossypium hirsutum diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn inducible enzyme Gossypium hirsutum diphosphate + RNAn+1
-
 ?
2.7.9.3 ATP + selenide + H2O
-
 Bos taurus AMP + selenophosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 GsOAS-TL1
-
 Glycine max
2.5.1.47 O-acetylserine(thiol)lyase
-
 Glycine max
2.5.1.47 OAS-TL
-
 Glycine max
2.7.7.48 GhRdRP
-
 Gossypium hirsutum
2.7.9.3 Bt1 SPS2 isoform 1 Bos taurus
2.7.9.3 selenophosphate synthetase 2
-
 Bos taurus
2.7.9.3 SEP synthetase
-
 Bos taurus
2.7.9.3 Sps2
-
 Bos taurus