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Literature summary extracted from

  • Buguliskis, J.S.; Casta, L.J.; Butz, C.E.; Matsumoto, Y.; Taraschi, T.F.
    Expression and biochemical characterization of Plasmodium falciparum DNA ligase I (2007), Mol. Biochem. Parasitol., 155, 128-137.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 KCl 30 mM, more than 60% reduction in product formation Plasmodium falciparum
6.5.1.1 NaCl above 20 mM, more than 60% reduction in product formation Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.0000026
-
 nicked DNA 37°C, pH 8.5 Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Ca2+ enzyme can utilize Ca2+ as cofactor Plasmodium falciparum
6.5.1.1 Mg2+ maximal activation at 0.6 mM Plasmodium falciparum
6.5.1.1 Mn2+ maximal activation at 6 mM Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m PfLigI is able to join RNA-DNA substrates only when the RNA sequence is upstream of the nick. Slight activity with dATP. No activity with NAD+, UTP, CTP and GTP. Slight activity with dATP. No activity with NAD+, UTP, CTP or GTP Plasmodium falciparum AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
6.5.1.1 ATP + nicked DNA
-
 Plasmodium falciparum AMP + diphosphate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 DNA ligase I
-
 Plasmodium falciparum
6.5.1.1 PfLigI
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.1 37
-
-
 Plasmodium falciparum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.5.1.1 25 40 about 50% of maximal activity at 25°C and at 40°C Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5.1.1 0.0023
-
 nicked DNA 37°C, pH 8.5 Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 8.5
-
-
 Plasmodium falciparum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.1 7.5 9.5 pH 7.5: about 40% of maximal activity, pH 9.5: about 40% of maximal activity Plasmodium falciparum