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Literature summary extracted from

  • Gagnon, D.; Foucher, A.; Girard, I.; Ouellette, M.
    Stage specific gene expression and cellular localization of two isoforms of the serine hydroxymethyltransferase in the protozoan parasite Leishmania (2006), Mol. Biochem. Parasitol., 150, 63-71.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.2.1 analysis protein sequence of the two isoforms, the short form SHMT-S and the long form SHMT-L, show 59% identity Leishmania major

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 both isoforms cloned in frame with EGFP at their C-terminus and transfected into Leishmania major Leishmania major

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 D-cycloserine overexpression of SHMT-S increases resistance in a rich folate containing medium Leishmania major
2.1.2.1 Thiosemicarbazide poorly active against promastigotes, but SHMT-S transfectants can provide a small but significant resistance Leishmania major

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytosol SHMT-S Leishmania major 5829
-
2.1.2.1 additional information SHMT-L in an organelle that has hallmarks of the parasite mitochondrion Leishmania major
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 50600
-
sequence analysis, SHMT-S Leishmania major
2.1.2.1 53100
-
sequence analysis, SHMT-L Leishmania major

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Leishmania infantum
-
-
-
2.1.2.1 Leishmania major
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.1 amastigote preferentially expressed Leishmania infantum
-
2.1.2.1 promastigote
-
Leishmania infantum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate adding 2fold more glycine in the medium increases significantly the expression of SHMT-S and to an even higher level, the expression of SHMT-L, adding 2fold more serine has the reverse effect on the expression of SHMT-L, while the expression of SHMT-S does not change significantly Leishmania infantum glycine + 5,10-methylenetetrahydrofolate + H2O
-
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Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
Leishmania major
2.1.2.1 serine hydroxymethyltransferase
-
Leishmania infantum
2.1.2.1 SHMT-L
-
Leishmania major
2.1.2.1 SHMT-L
-
Leishmania infantum
2.1.2.1 SHMT-S
-
Leishmania major
2.1.2.1 SHMT-S
-
Leishmania infantum