EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.2.1 | analysis | protein sequence of the two isoforms, the short form SHMT-S and the long form SHMT-L, show 59% identity | Leishmania major |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.1 | both isoforms cloned in frame with EGFP at their C-terminus and transfected into Leishmania major | Leishmania major |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | D-cycloserine | overexpression of SHMT-S increases resistance in a rich folate containing medium | Leishmania major | |
2.1.2.1 | Thiosemicarbazide | poorly active against promastigotes, but SHMT-S transfectants can provide a small but significant resistance | Leishmania major |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.2.1 | cytosol | SHMT-S | Leishmania major | 5829 | - |
2.1.2.1 | additional information | SHMT-L in an organelle that has hallmarks of the parasite mitochondrion | Leishmania major | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 50600 | - |
sequence analysis, SHMT-S | Leishmania major |
2.1.2.1 | 53100 | - |
sequence analysis, SHMT-L | Leishmania major |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.1 | Leishmania infantum | - |
- |
- |
2.1.2.1 | Leishmania major | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.2.1 | amastigote | preferentially expressed | Leishmania infantum | - |
2.1.2.1 | promastigote | - |
Leishmania infantum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | L-serine + tetrahydrofolate | adding 2fold more glycine in the medium increases significantly the expression of SHMT-S and to an even higher level, the expression of SHMT-L, adding 2fold more serine has the reverse effect on the expression of SHMT-L, while the expression of SHMT-S does not change significantly | Leishmania infantum | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.2.1 | serine hydroxymethyltransferase | - |
Leishmania major |
2.1.2.1 | serine hydroxymethyltransferase | - |
Leishmania infantum |
2.1.2.1 | SHMT-L | - |
Leishmania major |
2.1.2.1 | SHMT-L | - |
Leishmania infantum |
2.1.2.1 | SHMT-S | - |
Leishmania major |
2.1.2.1 | SHMT-S | - |
Leishmania infantum |