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Literature summary extracted from

  • Galazka, J.; Striepen, B.; Ullman, B.
    Adenosine kinase from Cryptosporidium parvum (2006), Mol. Biochem. Parasitol., 149, 223-230.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.20 CpAK gene codon-optimized for expression in Escherichia coli Cryptosporidium parvum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 2-chloroadenosine adenosine phosphorylation is inhibited by 100fold excess of the inhibitor Cryptosporidium parvum
2.7.1.20 4-nitro-6-benzylthioguanosine 0.2 mM, partial inhibition Cryptosporidium parvum
2.7.1.20 4-nitro-6-benzylthioinosine 0.2 mM, partial inhibition Cryptosporidium parvum
2.7.1.20 5'-amino-5'-deoxyadenosine adenosine phosphorylation is inhibited by 100fold excess of the inhibitor Cryptosporidium parvum
2.7.1.20 5'-deoxyadenosine adenosine phosphorylation is inhibited by 100fold excess of the inhibitor Cryptosporidium parvum
2.7.1.20 adenosine strongly inhibited by 100fold excess adenosine Cryptosporidium parvum
2.7.1.20 EDTA 100 mM, complete inhibition Cryptosporidium parvum
2.7.1.20 tubercidin adenosine phosphorylation is inhibited by 100fold excess of the inhibitor Cryptosporidium parvum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.0014
-
 adenosine 37°C, pH 8.0 Cryptosporidium parvum
2.7.1.20 0.041
-
 ATP 37°C, pH 8.0 Cryptosporidium parvum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Ca2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum
2.7.1.20 Co2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum
2.7.1.20 Fe2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum
2.7.1.20 Mg2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum
2.7.1.20 Mn2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum
2.7.1.20 Zn2+ activity is dependent on divalent cation. Activity is highest in presence of Mg2+ (5 mM) or Fe2+ (5 mM). 70% of maximal activation with Mn2+ (5 mM) or Ca2+ (5 mM). 35% of maximal activation with Co2+ (5 mM) or Zn2+ (5 mM) Cryptosporidium parvum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Cryptosporidium parvum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 recombinant Cryptosporidium parvum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + adenosine no phosphorylation of inosine or guanosine Cryptosporidium parvum ADP + AMP
-
 ?
2.7.1.20 CTP + adenosine about 60% of activity with ATP Cryptosporidium parvum CDP + AMP
-
 ?
2.7.1.20 GTP + adenosine about 50% of activity with ATP Cryptosporidium parvum GDP + AMP
-
 ?
2.7.1.20 TTP + adenosine about 10% of activity with ATP Cryptosporidium parvum TDP + AMP
-
 ?
2.7.1.20 UTP + adenosine about 40% of activity with ATP Cryptosporidium parvum UDP + AMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.20 CpAK
-
 Cryptosporidium parvum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.20 2.6
-
 ATP 37°C, pH 8.0 Cryptosporidium parvum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.20 7 8
-
 Cryptosporidium parvum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.20 5.5 10.5 pH 5.5: about 40% of maximal activity, pH 10.5: about 65% of maximal activity Cryptosporidium parvum