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Literature summary extracted from

  • Yap, T.L.; Xu, T.; Chen, Y.L.; Malet, H.; Egloff, M.P.; Canard, B.; Vasudevan, S.G.; Lescar, J.
    Crystal structure of the Dengue virus RNA-dependent RNA polymerase catalytic domain at 1.85-Angstrom resolution (2007), J. Virol., 81, 4753-4765.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 expressed in Escherichia coli BL21(DE3) cells Dengue virus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.48 hanging drop vapour diffusion method, using 0.1 M Tris-HCl at pH 8.5, 0.8 M potassium-sodium tartrate, 0.5% (w/v) PEG monomethyl ether 5000 Dengue virus
2.7.7.48 hanging-drop vapor diffusion method at 4°C, three-dimensional structure of a catalytically active DENV RdRp domain determined to 1.85 A resolution by X-ray crystallography Dengue virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 3'-dGTP
-
 Dengue virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Zn structure reveals the presence of two zinc ion binding motifs Dengue virus
2.7.7.48 Zn2+ contains zinc, Zn2+ likely contributes to the structural stability of the region near motif E of the DENV 3 polymerase Dengue virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.48 104000
-
 gel filtration Dengue virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn Dengue virus
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Dengue virus
-
-
-
2.7.7.48 Dengue virus Q6DLV0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 HisTrap HP column chromatography, 15S column chromatography, ultrafiltration, and Superdex 200 gel filtration Dengue virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Dengue virus diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn homopolymer C as the template and biotinoligo(G)20 as the primer Dengue virus diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn using homopolymer C as the template Dengue virus diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 DENV 3 RdRp
-
 Dengue virus
2.7.7.48 DENV RdRp
-
 Dengue virus
2.7.7.48 NS5 protein
-
 Dengue virus
2.7.7.48 NS5 protein bifunctional and contains 900 amino acids. The S-adenosyl methionine transferase activity resides within its N-terminal domain, and residues 270 to 900 form the RNA-dependent RNA polymerase (RdRp) catalytic domain Dengue virus
2.7.7.48 RNA-dependent RNA polymerase
-
 Dengue virus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.48 0.00022
-
-
 Dengue virus 3'-dGTP