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Literature summary extracted from

  • Marcotte, L.L.; Wass, A.B.; Gohara, D.W.; Pathak, H.B.; Arnold, J.J.; Filman, D.J.; Cameron, C.E.; Hogle, J.M.
    Crystal structure of poliovirus 3CD protein: virally encoded protease and precursor to the RNA-dependent RNA polymerase (2007), J. Virol., 81, 3583-3596.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 expressed in Escherichia coli BL21(DE3)/pCG1 cells Enterovirus C

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.48 crystal structure of poliovirus 3CD protein, a virally encoded protease and precursor to the RNA-dependent RNA polymerase Enterovirus C
2.7.7.48 hanging drop vapour diffusion method, with 2 M ammonium sulfate, 0.1 M HEPES (pH 7.0), and 0.3% Jeffamine M600 (pH 7.0) Enterovirus C

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.48 G1A mutant is only partially active and the N-terminal hydrogen-bonding network is partly disrupted Enterovirus C
2.7.7.48 G64S inactive Enterovirus C
2.7.7.48 L446D mutant with disrupt interface I of 3Dpol Enterovirus C
2.7.7.48 R455D mutant with disrupt interface I of 3Dpol Enterovirus C

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Enterovirus C
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 ammonium sulfate precipitation, Q-Sepharose column chromatography, and Superdex 200 gel filtration Enterovirus C

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Enterovirus C diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 3CD protein precursor of the RNA-dependent RNA polymerase, although 3CD is a fully functional protease, it lacks polymerase activity Enterovirus C
2.7.7.48 3Dpol
-
 Enterovirus C
2.7.7.48 RNA-dependent RNA polymerase
-
 Enterovirus C