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Literature summary extracted from

  • Fullerton, S.W.; Blaschke, M.; Coutard, B.; Gebhardt, J.; Gorbalenya, A.; Canard, B.; Tucker, P.A.; Rohayem, J.
    Structural and functional characterization of sapovirus RNA-dependent RNA polymerase (2007), J. Virol., 81, 1858-1871.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 fusion protein bearing a His6 tag at its C-terminus or at its N terminus is overexpressed in Escherichia coli Sapovirus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.48 overall structure of the sapovirus 3Dpol is determined by X-ray crystallography to 2.32 A resolution Sapovirus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mg2+ over a concentration range from 0.5 to 5 mM, sapovirus 3Dpol displays a clear preference for Mn2+ over Mg2+ Sapovirus
2.7.7.48 Mn2+ over a concentration range from 0.5 to 5 mM, sapovirus 3Dpol displays a clear preference for Mn2+ over Mg2+ Sapovirus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.48 57000
-
x * 57000, SDS-PAGE Sapovirus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn Sapovirus key enzyme of replication diphosphate + RNAn+1
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Sapovirus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn key enzyme of replication Sapovirus diphosphate + RNAn+1
-
?
2.7.7.48 nucleoside triphosphate + RNAn in a synthetic RNA template-dependent reaction, sapovirus 3Dpol synthesizes a double-stranded RNA or labels the template 3' terminus by terminal transferase activity. Initiation of RNA synthesis occurs de novo on heteropolymeric templates or in a primer dependent manner on polyadenylated templates Sapovirus diphosphate + RNAn+1
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.48 ? x * 57000, SDS-PAGE Sapovirus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 3Dpol-like protein the RNA-dependent RNA polymerase is associated with the 3Dpol-like protein Sapovirus