Literature summary extracted from
Fullerton, S.W.; Blaschke, M.; Coutard, B.; Gebhardt, J.; Gorbalenya, A.; Canard, B.; Tucker, P.A.; Rohayem, J.
Structural and functional characterization of sapovirus RNA-dependent RNA polymerase (2007), J. Virol., 81, 1858-1871.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.7.48 |
fusion protein bearing a His6 tag at its C-terminus or at its N terminus is overexpressed in Escherichia coli |
Sapovirus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.7.7.48 |
overall structure of the sapovirus 3Dpol is determined by X-ray crystallography to 2.32 A resolution |
Sapovirus |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.7.48 |
Mg2+ |
over a concentration range from 0.5 to 5 mM, sapovirus 3Dpol displays a clear preference for Mn2+ over Mg2+ |
Sapovirus |
|
2.7.7.48 |
Mn2+ |
over a concentration range from 0.5 to 5 mM, sapovirus 3Dpol displays a clear preference for Mn2+ over Mg2+ |
Sapovirus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.7.48 |
57000 |
- |
x * 57000, SDS-PAGE |
Sapovirus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.7.7.48 |
nucleoside triphosphate + RNAn |
Sapovirus |
key enzyme of replication |
diphosphate + RNAn+1 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.48 |
Sapovirus |
- |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.48 |
nucleoside triphosphate + RNAn |
key enzyme of replication |
Sapovirus |
diphosphate + RNAn+1 |
- |
? |
|
2.7.7.48 |
nucleoside triphosphate + RNAn |
in a synthetic RNA template-dependent reaction, sapovirus 3Dpol synthesizes a double-stranded RNA or labels the template 3' terminus by terminal transferase activity. Initiation of RNA synthesis occurs de novo on heteropolymeric templates or in a primer dependent manner on polyadenylated templates |
Sapovirus |
diphosphate + RNAn+1 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.7.48 |
? |
x * 57000, SDS-PAGE |
Sapovirus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.7.48 |
3Dpol-like protein |
the RNA-dependent RNA polymerase is associated with the 3Dpol-like protein |
Sapovirus |