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Literature summary extracted from

  • Cao, P.; Gong, Y.; Tang, L.; Leung, Y.C.; Jiang, T.
    Crystal structure of human pyridoxal kinase (2006), J. Struct. Biol., 154, 327-332.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.35 expressed in Escherichia coli strain BL21 (DE3) plysS Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.35 hanging drop vapour diffusion method using 0.1 mM Tris-HCl pH 8.0, 1.8 M NH4Ac, and 3% glycol Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.35 Homo sapiens O00764
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.35 Ni-NTA agarose column chromatography and HiLoad 26/60 Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.35 ATP + pyridoxal
-
 Homo sapiens ADP + pyridoxal 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.35 homodimer x-ray crystallography Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.35 HPLK
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.35 ATP
-
 Homo sapiens