Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Oishi, H.; Ebina, M.
    Isolation of cDNA and enzymatic properties of betaine aldehyde dehydrogenase from Zoysia tenuifolia (2005), J. Plant Physiol., 162, 1077-1086.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.8 into the pCR2.1-TOPO and pGEX-6p-2 vector for sequencing of the coding region and expression of the enzyme in Escherichia coli BL21, respectively Zoysia tenuifolia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.8 NaCl 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity; 100 mM about 70% activity to 500 mM about 40% activity Zoysia tenuifolia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.8 0.004
-
 3-aminopropionaldehyde
-
 Zoysia tenuifolia
1.2.1.8 0.049
-
 4-Aminobutyraldehyde
-
 Zoysia tenuifolia
1.2.1.8 0.291
-
 Betaine aldehyde
-
 Zoysia tenuifolia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.8 55500
-
 estimated by SDS-PAGE Zoysia tenuifolia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Zoysia tenuifolia
-
 glycine betaine + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Zoysia tenuifolia Q6BD86 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
1.2.1.8 Zoysia tenuifolia Q6BD88 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
1.2.1.8 Zoysia tenuifolia Q6BD90 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
1.2.1.8 Zoysia tenuifolia Q6BD91 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
1.2.1.8 Zoysia tenuifolia Q6BD93 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
1.2.1.8 Zoysia tenuifolia Q6BD95 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
1.2.1.8 Zoysia tenuifolia Q6BD99 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
1.2.1.8 Zoysia tenuifolia Q6BDA3 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-
1.2.1.8 Zoysia tenuifolia Q6BDA4 positive clone isolated after a cDNA library screening coding for a betaine aldehyde dehydrogenase isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8 on GSTrap FF columns Zoysia tenuifolia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 3-aminopropionaldehyde + NAD+ + H2O
-
 Zoysia tenuifolia 3-aminopropionic acid + NADH + H+
-
 ?
1.2.1.8 4-aminobutyraldehyde + NAD+ + H2O
-
 Zoysia tenuifolia 4-aminobutyric acid + NADH + H+
-
 ?
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
 Zoysia tenuifolia glycine betaine + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.8 BADH
-
 Zoysia tenuifolia
1.2.1.8 betaine aldehyde dehydrogenase
-
 Zoysia tenuifolia
1.2.1.8 ZBD1
-
 Zoysia tenuifolia
1.2.1.8 Zoysia betaine aldehyde dehydrogenase 1
-
 Zoysia tenuifolia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.8 25
-
 activity assay Zoysia tenuifolia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.8 8
-
 activity assay Zoysia tenuifolia
1.2.1.8 9.5
-
 measurement of enzyme activity in dependence on pH, activity is strongly affected by the used buffer at the same pH Zoysia tenuifolia

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+
-
 Zoysia tenuifolia