BRENDA - Enzyme Database show

Petrosamine B, an inhibitor of the Helicobacter pylori enzyme aspartyl semialdehyde dehydrogenase from the Australian sponge Oceanapia sp

Carroll, A.R.; Ngo, A.; Quinn, R.J.; Redburn, J.; Hooper, J.N.; J. Nat. Prod. 68, 804-806 (2005)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.2.1.11
medicine
target for antibiotic development
Helicobacter pylori
Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.11
expression in Escherichia coli
Helicobacter pylori
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
petrosamine B
50% inhibition at 0.306 mM. Petrosamine B is a pyridoacridine alkaloid isolated from the sponge Oceanapia sp.; isolated from the methanol extract of the saustralian sponge Oceanapia sp.
Helicobacter pylori
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Helicobacter pylori
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Helicobacter pylori
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
Ni-affinity chromatography
Helicobacter pylori
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
675505
Helicobacter pylori
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
23
-
activity assay
Helicobacter pylori
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
8
-
activity assay
Helicobacter pylori
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Helicobacter pylori
1.2.1.11
NADPH
-
Helicobacter pylori
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.2.1.11
0.306
-
-
Helicobacter pylori
petrosamine B
Application (protein specific)
EC Number
Application
Commentary
Organism
1.2.1.11
medicine
target for antibiotic development
Helicobacter pylori
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
expression in Escherichia coli
Helicobacter pylori
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Helicobacter pylori
1.2.1.11
NADPH
-
Helicobacter pylori
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.2.1.11
0.306
-
-
Helicobacter pylori
petrosamine B
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
petrosamine B
50% inhibition at 0.306 mM. Petrosamine B is a pyridoacridine alkaloid isolated from the sponge Oceanapia sp.; isolated from the methanol extract of the saustralian sponge Oceanapia sp.
Helicobacter pylori
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Helicobacter pylori
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
Ni-affinity chromatography
Helicobacter pylori
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
675505
Helicobacter pylori
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
23
-
activity assay
Helicobacter pylori
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
8
-
activity assay
Helicobacter pylori