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Literature summary extracted from

  • Weyand, S.; Kefala, G.; Weiss, M.S.
    The three-dimensional structure of N-succinyldiaminopimelate aminotransferase from Mycobacterium tuberculosis (2007), J. Mol. Biol., 367, 825-838.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.17 expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.17 diffration to 2.0 A resolution. The orthorhombic crystals contain one functional dimer in the asymmetric unit. The homodimer displays the typical S-shape of class I pyridoxal 5'-phosphate-binding proteins. The two active sites of the dimer both feature an internal aldimine with the co-factor pyridoxal 5'-phosphate covalently bound to the Lys232, although neither substrate nor cofactor has been added during protein production, purification and crystallization. Nine water molecules are conserved in the active site and form an intricate hydrogen-bonding network with the co-factor and the surrounding amino acid residues Mycobacterium tuberculosis
2.6.1.17 sitting drop vapour diffusion method, in the presence of 20% (w/v) PEG-3350, Tris-HCl (pH 8.5) and 200 mM MgCl2 Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.17 Mycobacterium tuberculosis P9WPZ5
-
-
2.6.1.17 Mycobacterium tuberculosis H37Rv P9WPZ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.17 affinity column chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.17 N-succinyl-2-amino-6-oxoheptanedioate + L-glutamate
-
 Mycobacterium tuberculosis N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate
-
 ?
2.6.1.17 N-succinyl-2-amino-6-oxoheptanedioate + L-glutamate
-
 Mycobacterium tuberculosis H37Rv N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.17 dimer crystallization data Mycobacterium tuberculosis
2.6.1.17 homodimer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.6.1.17 DAP-AT
-
 Mycobacterium tuberculosis
2.6.1.17 DapC
-
 Mycobacterium tuberculosis
2.6.1.17 Rv0858c
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.17 pyridoxal 5'-phosphate
-
 Mycobacterium tuberculosis