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Literature summary extracted from

  • Milani, M.; Balconi, E.; Aliverti, A.; Mastrangelo, E.; Seeber, F.; Bolognesi, M.; Zanetti, G.
    Ferredoxin-NADP(+) reductase from Plasmodium falciparum undergoes NADP(+)-dependent dimerization and inactivation: functional and crystallographic analysis (2007), J. Mol. Biol., 367, 501-513.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 expressed in Escherichia coli Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.18.1.2 free enzyme and in complex with 2’-phospho-AMP. Structures reveal a covalent dimer, which relies on the oxidation of residue C99 in two opposing subunits, and a helix-coil transition that occurs in the NADP-binding domain, triggered by 2’-phospho-AMP binding. Studies in solution show that NADP+, as well as 2’-phospho-AMP, promotes the formation of the disulfide-stabilized dimer. The dimer is inactive, but full activity is recovered upon disulfide reduction Plasmodium falciparum
1.18.1.2 in free form and in complex with 2'-phospho-AMP, in 22-25% PEG 4000, 0.1 M sodium cacodylate (pH 6.0), 0.2 M sodium acetate Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.1.2 ferricyanide
-
 Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.036
-
 NADPH 25°C, pH 8.2 Plasmodium falciparum
1.18.1.2 0.036
-
 NADPH with K3Fe(CN)6 as cosubstrate, at 25°C, in 100 mM Tris-HCl (pH 8.2) Plasmodium falciparum
1.18.1.2 0.095
-
 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride at 25°C in 200 mM Tris-HCl (pH 9.0), containing 70 mM NaCl and 0.1% Triton-X100 Plasmodium falciparum
1.18.1.2 0.35
-
 NADPH cosubstrate 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride, 25°C, pH 9.0 Plasmodium falciparum
1.18.1.2 0.35
-
 NADPH with 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride as cosubstrate, at 25°C, in 100 mM Tris-HCl (pH 8.2) Plasmodium falciparum
1.18.1.2 0.72
-
 NADH 25°C, pH 8.2 Plasmodium falciparum
1.18.1.2 0.72
-
 NADH with K3Fe(CN)6 as cosubstrate, at 25°C, in 100 mM Tris-HCl (pH 8.2) Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.2 apicoplast
-
 Plasmodium falciparum 20011
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 28000
-
 gel filtration Plasmodium falciparum
1.18.1.2 37270
-
 calculated from amino acid sequence Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Plasmodium falciparum C6KT68
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 2 ferricyanide + NADH
-
 Plasmodium falciparum 2 ferrocyanide + NAD+ + H+
-
 ?
1.18.1.2 2 ferricyanide + NADPH
-
 Plasmodium falciparum 2 ferrocyanide + NADP+ + H+
-
 ?
1.18.1.2 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride + NADPH
-
 Plasmodium falciparum ? + NADP+
-
 ?
1.18.1.2 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride + NADPH
-
 Plasmodium falciparum ?
-
 ?
1.18.1.2 K3Fe(CN)6 + NAD(P)H
-
 Plasmodium falciparum ?
-
 ?
1.18.1.2 reduced ferredoxin + NADP+
-
 Plasmodium falciparum oxidized ferredoxin + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 dimer the enzyme undergoes NADP+-dependent dimerization (inactive enzyme form) Plasmodium falciparum
1.18.1.2 monomer 1 * 28000, gel filtration Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 Ferredoxin-NADP(+) reductase
-
 Plasmodium falciparum
1.18.1.2 FNR
-
 Plasmodium falciparum

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 NADP+
-
 Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.18.1.2 0.185
-
 ferricyanide 25°C, pH 8.2, cosubstrate NADH Plasmodium falciparum
1.18.1.2 0.23
-
 ferricyanide 25°C, pH 8.2, cosubstrate NADPH Plasmodium falciparum