Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Di Luccio, E.; Petschacher, B.; Voegtli, J.; Chou, H.T.; Stahlberg, H.; Nidetzky, B.; Wilson, D.K.
    Structural and kinetic studies of induced fit in xylulose kinase from Escherichia coli (2007), J. Mol. Biol., 365, 783-798.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.17 hanging drop vapour diffusion method using 1.5 M ammonium sulfate, 50 mM sodium citrate, pH 6, 1% (w/v) t-butanol and 25% (v/v) ethylene glycol, at 25°C Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.17 D233A loss of activity Escherichia coli
2.7.1.17 D6A 19fold decreased ATPase activity compared to the wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.17 5-fluoro-xylulose 0.18 mM, strong inhibition of the ATPase activity of xylulokinase Escherichia coli
2.7.1.17 AMPPNP
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.17 0.29
-
 D-xylulose wild type enzyme Escherichia coli
2.7.1.17 8.2
-
 ATP mutant D233A Escherichia coli
2.7.1.17 14
-
 D-ribulose wild type enzyme Escherichia coli
2.7.1.17 127
-
 xylitol wild type enzyme Escherichia coli
2.7.1.17 141
-
 D-arabitol wild type enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.17 55000
-
 2 * 55000, dynamic light scattering Escherichia coli
2.7.1.17 110000
-
 dynamic light scattering Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.17 Escherichia coli P09099
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.17 ATP + D-arabitol
-
 Escherichia coli ADP + D-arabitol 5-phosphate
-
 ?
2.7.1.17 ATP + D-ribulose
-
 Escherichia coli ADP + D-ribulose 5-phosphate
-
 ?
2.7.1.17 ATP + D-xylulose
-
 Escherichia coli ADP + D-xylulose 5-phosphate
-
 r
2.7.1.17 ATP + xylitol
-
 Escherichia coli ADP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.17 dimer 2 * 55000, dynamic light scattering Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.17 0.0038
-
 ATP mutant D6A Escherichia coli
2.7.1.17 0.13
-
 ATP mutant D233A Escherichia coli
2.7.1.17 105
-
 D-arabitol wild type enzyme Escherichia coli
2.7.1.17 235
-
 D-ribulose wild type enzyme Escherichia coli
2.7.1.17 237
-
 xylitol wild type enzyme Escherichia coli
2.7.1.17 255
-
 D-xylulose wild type enzyme Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.17 ATP
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.17 0.026
-
 5-fluoro-xylulose in the presence of 5 mM ATP Escherichia coli
2.7.1.17 0.15
-
 5-fluoro-xylulose in the presence of 0.167 mM ATP Escherichia coli
2.7.1.17 0.71
-
 AMPPNP in the presence of 4.25 mM xylulose Escherichia coli
2.7.1.17 1.1
-
 AMPPNP in the presence of 0.28 mM xylulose Escherichia coli
2.7.1.17 1.9
-
 AMPPNP in the presence of 0.167 mM ATP Escherichia coli