BRENDA - Enzyme Database show

A new branch in the family: structure of aspartate-beta-semialdehyde dehydrogenase from Methanococcus jannaschii

Faehnle, C.R.; Ohren, J.F.; Viola, R.E.; J. Mol. Biol. 353, 1055-1068 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.11
expression in Escherichia coli; into the pET41-41a vector for transformation into Rosetta-competent Escherichia coli cells
Methanocaldococcus jannaschii
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.2.1.11
2.3 A resolution; the structure of aspartate-beta-semialdehyde dehydrogenase has been determined to 2.3 A resolution using multiwavelength anomalous diffraction phasing of a seleno-methionine-substituted derivative
Methanocaldococcus jannaschii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
2'-phosphoribose AMP
-
Methanocaldococcus jannaschii
1.2.1.11
ADP
-
Methanocaldococcus jannaschii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.013
-
NADP+
-
Methanocaldococcus jannaschii
1.2.1.11
1.7
-
NAD+
-
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Methanocaldococcus jannaschii
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Methanocaldococcus jannaschii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
anion exchange chromatography and gel-filtration; using anion-exchange chromatography, Source 15Q, followed by gel filtration
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NAD+
-
675355
Methanocaldococcus jannaschii
L-4-aspartyl phosphate + NADH + H+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
675355
Methanocaldococcus jannaschii
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.11
homodimer
crystallization
Methanocaldococcus jannaschii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Methanocaldococcus jannaschii
1.2.1.11
NADPH
-
Methanocaldococcus jannaschii
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.11
0.05
-
2'-phosphoribose AMP
-
Methanocaldococcus jannaschii
1.2.1.11
0.4
-
ADP
-
Methanocaldococcus jannaschii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
expression in Escherichia coli; into the pET41-41a vector for transformation into Rosetta-competent Escherichia coli cells
Methanocaldococcus jannaschii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Methanocaldococcus jannaschii
1.2.1.11
NADPH
-
Methanocaldococcus jannaschii
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.2.1.11
2.3 A resolution; the structure of aspartate-beta-semialdehyde dehydrogenase has been determined to 2.3 A resolution using multiwavelength anomalous diffraction phasing of a seleno-methionine-substituted derivative
Methanocaldococcus jannaschii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
2'-phosphoribose AMP
-
Methanocaldococcus jannaschii
1.2.1.11
ADP
-
Methanocaldococcus jannaschii
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.11
0.05
-
2'-phosphoribose AMP
-
Methanocaldococcus jannaschii
1.2.1.11
0.4
-
ADP
-
Methanocaldococcus jannaschii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.013
-
NADP+
-
Methanocaldococcus jannaschii
1.2.1.11
1.7
-
NAD+
-
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Methanocaldococcus jannaschii
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
anion exchange chromatography and gel-filtration; using anion-exchange chromatography, Source 15Q, followed by gel filtration
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NAD+
-
675355
Methanocaldococcus jannaschii
L-4-aspartyl phosphate + NADH + H+
-
-
-
?
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
675355
Methanocaldococcus jannaschii
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.11
homodimer
crystallization
Methanocaldococcus jannaschii