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Literature summary extracted from
- Heme catalyzes tyrosine 385 nitration and inactivation of prostaglandin H2 synthase-1 by peroxynitrite (2006), J. Lipid Res., 47, 898-911.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.1 | peroxynitrite | peroxidase activity, MS-analysis reveals that tyrosine 385 is a target for nitration by ONOO- only when heme is present | Ovis aries |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.99.1 | - |
Ovis aries |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.1 | aspirin | cyclooxygenase activity | Ovis aries | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.99.1 | microsome | - |
Ovis aries | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 70000 | - |
confirmed by SDS-PAGE, Western blot analysis and MALDI-TOF | Ovis aries |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.1 | arachidonate + AH2 + O2 | Ovis aries | - |
prostaglandin H2 + A + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.1 | Ovis aries | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.99.1 | N-octyl-beta-D-glucopyranoside, Tween-20, CHAPS, and maltoside are used for solubilization of prostaglandin H2 synthase-1, frozen microsomal extracts are thawed and purified using a fast-flow DEAE-Sepharose and a S-300 column | Ovis aries |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.99.1 | seminal vesicle | - |
Ovis aries | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.1 | arachidonate + AH2 + O2 | - |
Ovis aries | prostaglandin H2 + A + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.99.1 | PGHS-1 | - |
Ovis aries |
| 1.14.99.1 | prostaglandin H2 synthase-1 | - |
Ovis aries |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 25 | - |
activity assay | Ovis aries |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 8 | - |
activity assay | Ovis aries |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.1 | heme | hemin and hematin | Ovis aries | |
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Release 2023.1 (January 2023)
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