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Literature summary extracted from

  • Bukh, C.; Lund, M.; Bjerrum, M.J.
    Kinetic studies on the reaction between Trametes villosa laccase and dioxygen (2006), J. Inorg. Biochem., 100, 1547-1557.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 gene lcc1, expression in Aspergillus oryzae as precursor enzyme Trametes villosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
 additional information transient/stopped-flow and steady-state kinetics, modeling Trametes villosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ blue multi-copper oxidase Trametes villosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 63000
-
 2 * 63000, recombinant enzyme, SDS-PAGE Trametes villosa

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trametes villosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 recombinant enzyme from Aspergillus oryzae by gel filtration, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, and ultrafiltration Trametes villosa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.10.3.2 4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O reaction mechanism of the reduction of dioxygen to water, second-order rate constant for the reaction with dioxygen, ping-pong mechanism, kinetic model Trametes villosa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 4-hydroxybenzoic acid + O2
-
 Trametes villosa ?
-
 ?
1.10.3.2 4-hydroxyphenylacetic acid + O2 second-order rate constant for the reaction with dioxygen Trametes villosa ?
-
 ?
1.10.3.2 guaiacolsulfonic acid + O2
-
 Trametes villosa ?
-
 ?
1.10.3.2 hydroquinone + O2
-
 Trametes villosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 dimer 2 * 63000, recombinant enzyme, SDS-PAGE Trametes villosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 25
-
 assay at Trametes villosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 10 35
-
 Trametes villosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5
-
 assay at Trametes villosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Trametes villosa isoelectric focusing
-
 3.5