BRENDA - Enzyme Database

Betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway

Kharbanda, K.K.; Mailliard, M.E.; Baldwin, C.R.; Beckenhauer, H.C.; Sorrell, M.F.; Tuma, D.J.; J. Hepatol. 46, 314-321 (2007)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.17
betaine
attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, the effect is enhanced by ethanol, which alone has no effect on expression levels of the enzyme, overview
Rattus norvegicus
2.1.1.17
ethanol
enhances the inducing and protecting effects of betaine on the enzyme, alone has only a slightly activating effect on the enzyme activity, overview
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.17
3 S-adenosyl-L-methionine + phosphatidylethanolamine
Rattus norvegicus
betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, overview
3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.17
Rattus norvegicus
Q08388
male Wistar rats
-
2.1.1.17
Rattus norvegicus
-
male Wistar rats weighing 180-200 g. 30-40% less phosphatidylcholine is generated in hepatocytes from ethanol-fed rats as compared to controls. Hepatocytes isolated from rats fed the betaine-supplemented ethanol diet have significantly higher phosphatidylcholine generation compared to hepatocytes from ethanol-fed rats.
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.17
hepatocyte
;
Rattus norvegicus
-
2.1.1.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.17
additional information
-
there is a significant positive linear relationship between phosphatidylethanolamine N-methyltransferase activity and S-adenosylmethionine:S-adenosylhomocysteine ratio (r = 0.45). There is a significant inverse relationship between phosphatidylethanolamine N-methyltransferase activity and triglycerids. The metabolic conversion of radiolabeled phosphatidylethanolamine to phosphatidylcholine over 3 h incubation period is taken as a measure of phosphatidylethanolamine N-methyltransferase.
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.17
3 S-adenosyl-L-methionine + phosphatidylethanolamine
-
675185
Rattus norvegicus
3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+
-
-
-
?
2.1.1.17
3 S-adenosyl-L-methionine + phosphatidylethanolamine
betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, overview
675185
Rattus norvegicus
3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+
-
-
-
?
2.1.1.17
additional information
the total hepatic S-adenosylmethionine levels remain unchanged by ethanol consumption for 4 weeks
675185
Rattus norvegicus
additional information
S-adenosylhomocysteine levels increase by ethanol consumption for 4 weeks. The S-adenosylmethionine:S-adenosylhomocysteine ratios are significantly lower in ethanol fed rats. Supplementation of betaine in the ethanol diet increases the hepatocellular S-adenosylmethionine:S-adenosylhomocysteine ratios to values that are statistically similar to those from rats fed the regular control diet. There is an significant inverse relationship between triglycerids and S-adenosylmethionine:S-adenosylhomocysteine ratio (r = - 0.36).
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.17
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.17
7.4
-
assay at
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.17
S-adenosyl-L-methionine
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.17
betaine
attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, the effect is enhanced by ethanol, which alone has no effect on expression levels of the enzyme, overview
Rattus norvegicus
2.1.1.17
ethanol
enhances the inducing and protecting effects of betaine on the enzyme, alone has only a slightly activating effect on the enzyme activity, overview
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.17
S-adenosyl-L-methionine
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.17
3 S-adenosyl-L-methionine + phosphatidylethanolamine
Rattus norvegicus
betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, overview
3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.17
hepatocyte
-
Rattus norvegicus
-
2.1.1.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.17
additional information
-
there is a significant positive linear relationship between phosphatidylethanolamine N-methyltransferase activity and S-adenosylmethionine:S-adenosylhomocysteine ratio (r = 0.45). There is a significant inverse relationship between phosphatidylethanolamine N-methyltransferase activity and triglycerids. The metabolic conversion of radiolabeled phosphatidylethanolamine to phosphatidylcholine over 3 h incubation period is taken as a measure of phosphatidylethanolamine N-methyltransferase.
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.17
3 S-adenosyl-L-methionine + phosphatidylethanolamine
-
675185
Rattus norvegicus
3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+
-
-
-
?
2.1.1.17
3 S-adenosyl-L-methionine + phosphatidylethanolamine
betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, overview
675185
Rattus norvegicus
3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+
-
-
-
?
2.1.1.17
additional information
the total hepatic S-adenosylmethionine levels remain unchanged by ethanol consumption for 4 weeks
675185
Rattus norvegicus
additional information
S-adenosylhomocysteine levels increase by ethanol consumption for 4 weeks. The S-adenosylmethionine:S-adenosylhomocysteine ratios are significantly lower in ethanol fed rats. Supplementation of betaine in the ethanol diet increases the hepatocellular S-adenosylmethionine:S-adenosylhomocysteine ratios to values that are statistically similar to those from rats fed the regular control diet. There is an significant inverse relationship between triglycerids and S-adenosylmethionine:S-adenosylhomocysteine ratio (r = - 0.36).
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.17
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.17
7.4
-
assay at
Rattus norvegicus