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Literature summary extracted from

  • Knoch, M.; van Pee, K.H.; Vining, L.C.; Lingens, F.
    Purification, properties and immunological detection of a bromoperoxidase-catalase from Streptomyces venezuelae and from a chloramphenicol-nonproducing mutant (1989), J. Gen. Microbiol., 135, 2493-2502.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.11.1.18 after treatment with a mixture of chloroform/ethanol the enzyme still shows 65% of its initial brominating activity Streptomyces venezuelae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.18 3-Amino-1,2,4-triazole 45% inhibition at 5 mM, 90% inhibition at 40 mM Streptomyces venezuelae
1.11.1.18 hydroxylamine 0.05 mM, 50% inhibition of brominating activity Streptomyces venezuelae
1.11.1.18 potassium cyanide 0.05 mM, complete inhibition of bromoperoxidase-catalase activity Streptomyces venezuelae
1.11.1.18 Sodium azide 0.05 mM, complete inhibition of bromoperoxidase-catalase activity Streptomyces venezuelae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.18 61000
-
 2 * 61000, SDS-PAGE Streptomyces venezuelae
1.11.1.18 127000 136000 gel filtration Streptomyces venezuelae

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.18 Streptomyces venezuelae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.18
-
 Streptomyces venezuelae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.18 1.77
-
 bromoperoxidase activity Streptomyces venezuelae
1.11.1.18 963
-
 catalase activity Streptomyces venezuelae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.18 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Streptomyces venezuelae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.18 dimer 2 * 61000, SDS-PAGE Streptomyces venezuelae

Synonyms

EC Number Synonyms Comment Organism
1.11.1.18 bromoperoxidase-catalase
-
 Streptomyces venezuelae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.18 4.75
-
 bromination of 1,1-dimethyl-4-chloro-3,5-cyclohexanedione Streptomyces venezuelae
1.11.1.18 7 8.5 catalase activity Streptomyces venezuelae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.18 Streptomyces venezuelae isoelectric focusing, pH-range: 3.5-9.5
-
 4.5