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Literature summary extracted from
- Purification and partial characterization of multiple bromoperoxidases from Streptomyces griseus (1988), J. Gen. Microbiol., 134, 3141-3149.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.18 | 0.002 | - |
1,1-dimethyl-4-chloro-3,5-cyclohexanedione | nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus |  |
| 1.11.1.18 | 0.0079 | - |
1,1-dimethyl-4-chloro-3,5-cyclohexanedione | nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus | |
| 1.11.1.18 | 6.4 | - |
Br- | nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus | |
| 1.11.1.18 | 95 | - |
H2O2 | nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus | |
| 1.11.1.18 | 160 | - |
Br- | nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus | |
| 1.11.1.18 | 430 | - |
H2O2 | nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.18 | 31000 | - |
3 * 31000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus |
| 1.11.1.18 | 43000 | - |
2 * 43000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 1b | Streptomyces griseus |
| 1.11.1.18 | 70000 | - |
gel filtration, nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus |
| 1.11.1.18 | 90000 | - |
gel filtration, nonhaem-type bromoperoxidase BPO 1b | Streptomyces griseus |
| 1.11.1.18 | 90000 | - |
gel filtration, nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.18 | Streptomyces griseus | - |
strain Tü 6 | - |
| 1.11.1.18 | Streptomyces griseus Tu6 | - |
strain Tü 6 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.18 | nonhaem-type bromoperoxidase BPO1a | Streptomyces griseus |
| 1.11.1.18 | nonhaem-type bromoperoxidase BPO1b | Streptomyces griseus |
| 1.11.1.18 | nonhaem-type bromoperoxidase BPO3 | Streptomyces griseus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.18 | additional information | - |
- |
Streptomyces griseus |
| 1.11.1.18 | 1.1 | - |
nonhaem-type bromoperoxidase BPO3 | Streptomyces griseus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.18 | Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Streptomyces griseus | ? | - |
? | |
| 1.11.1.18 | Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Streptomyces griseus Tu6 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.1.18 | dimer | 2 * 3400, SDS-PAGE, nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus |
| 1.11.1.18 | dimer | 2 * 43000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 1b | Streptomyces griseus |
| 1.11.1.18 | trimer | 3 * 31000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.18 | nonhaem-type bromoperoxidase BPO 1a | - |
Streptomyces griseus |
| 1.11.1.18 | nonhaem-type bromoperoxidase BPO 1b | - |
Streptomyces griseus |
| 1.11.1.18 | nonhaem-type bromoperoxidase BPO 3 | - |
Streptomyces griseus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.18 | 40 | - |
nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus |
| 1.11.1.18 | 60 | - |
nonhaem-type bromoperoxidase BPO3 | Streptomyces griseus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.18 | 60 | - |
50 min, complete inactivation, nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus |
| 1.11.1.18 | 60 | - |
50 min, no loss of activity, nonhaem-type bromoperoxidase BPO 1b | Streptomyces griseus |
| 1.11.1.18 | 60 | - |
50 min, no loss of activity, nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.18 | 4.5 | - |
nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus |
| 1.11.1.18 | 4.5 | - |
nonhaem-type bromoperoxidase BPO3, at low concentrations of H2O2 (1.6 mM) the pH-optimum shifts from pH 4.5 to pH 4.0 | Streptomyces griseus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.11.1.18 | Streptomyces griseus | isoelectric focusing, pH-range 3.5-9.5, nonhaem-type bromoperoxidase BPO 3 | - |
3.6 |
| 1.11.1.18 | Streptomyces griseus | isoelectric focusing, pH-range 3.5-9.5, nonhaem-type bromoperoxidase BPO 1a | - |
4.6 |
| 1.11.1.18 | Streptomyces griseus | isoelectric focusing, pH-range 3.5-9.5, nonhaem-type bromoperoxidase BPO 1b | - |
4.7 |
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