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Literature summary extracted from

  • Ni Eidhinn, D.M.; Murphy, E.; O'Beirne, D.
    Polyphenol oxidase from apple (Malus domestica Borkh. cv Bramley’s seedling): purification strategies and characterization (2006), J. Food Sci., 71, C51-C58.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Malus domestica
-
 cv. BramleyÂ’s seedling
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 native enzyme 75.5fold to homogeneity from apples purification in presence of AEBSF and aprotinin by ammonium sulfate fractionation, dialysis, gel filtration, and hydrophobic interaction chromatography, followed by anion exchange and hydroxyapatite chromatography Malus domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 fruit ripe Malus domestica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 227700
-
 highly purified enzyme, substrate catechol Malus domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 (-)-epicatechin + O2
-
 Malus domestica ?
-
 ?
1.10.3.1 4-methylcatechol + O2
-
 Malus domestica 4-methyl-1,2-benzoquinone + H2O
-
 ?
1.10.3.1 caffeic acid + O2
-
 Malus domestica ?
-
 ?
1.10.3.1 catechol + 1/2 O2
-
 Malus domestica 1,2-benzoquinone + H2O
-
 ?
1.10.3.1 DL-DOPA + O2
-
 Malus domestica dopaquinone + H2O
-
 ?
1.10.3.1 additional information substrate specificity of the purified enzyme, overview, highest PPO activity occurs with 4-methylcatechol, followed by catechol, pyrogallol, (-)-epicatechin, caffeic acid, and DL-dopa, little or no activity is detected toward the monophenolic compounds ferulic acid, L-tyrosine, and phenol Malus domestica ?
-
 ?
1.10.3.1 pyrogallol + O2
-
 Malus domestica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 polyphenol oxidase
-
 Malus domestica
1.10.3.1 PPO
-
 Malus domestica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 30
-
 with substrate catechol Malus domestica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.1 20 80
-
 Malus domestica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 20 30 72 h, purified enzyme, over 50% activity remaining Malus domestica
1.10.3.1 40 50 24 h, purified enzyme, over 50% activity remaining Malus domestica
1.10.3.1 60
-
 1 h, purified enzyme, over 50% activity remaining Malus domestica
1.10.3.1 70
-
 15 min, purified enzyme, over 50% activity remaining Malus domestica
1.10.3.1 80
-
 15 min, purified enzyme, inactivation Malus domestica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 6.5
-
 with substrate catechol Malus domestica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.1 2.5 9
-
 Malus domestica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.1 2.5 3.5 purified enzyme, most unstable at, less than 10% activity remaining after 2 h Malus domestica
1.10.3.1 4.5
-
 purified enzyme, over 50% activity reamining after 24 h Malus domestica
1.10.3.1 5 7.5 purified enzyme, over 50% activity reamining after 48 h Malus domestica
1.10.3.1 5.6 6.5 purified enzyme, most stable at, over 50% remaining activity after 72 h Malus domestica
1.10.3.1 8 8.5 purified enzyme, over 50% activity reamining after 24 h Malus domestica
1.10.3.1 9
-
 purified enzyme, over 50% activity reamining after 4 h Malus domestica