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Literature summary extracted from

  • Guelcin, I.; Kuefrevioglu, O.I.; Oktay, M.
    Purification and characterization of polyphenol oxidase from nettle (Urtica dioica L.) and inhibitory effects of some chemicals on enzyme activity (2005), J. Enzyme Inhib. Med. Chem., 20, 297-302.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 2-mercaptoethanol competitive Urtica dioica
1.10.3.1 benzoic acid noncompetitive Urtica dioica
1.10.3.1 glutathione competitive Urtica dioica
1.10.3.1 L-ascorbic acid competitive Urtica dioica
1.10.3.1 L-cysteine chloride competitive Urtica dioica
1.10.3.1 salicylic acid uncompetitive Urtica dioica
1.10.3.1 Sodium azide noncompetitive Urtica dioica
1.10.3.1 Sodium cyanide noncompetitive Urtica dioica
1.10.3.1 Sodium diethyl dithiocarbamate cstrong competitive inhibitor Urtica dioica
1.10.3.1 Sodium metabisulfite competitive Urtica dioica
1.10.3.1 Thiourea competitive Urtica dioica
1.14.18.1 2-mercaptoethanol competitive Urtica dioica
1.14.18.1 benzoic acid noncompetitive Urtica dioica
1.14.18.1 glutathione competitive Urtica dioica
1.14.18.1 L-ascorbic acid competitive Urtica dioica
1.14.18.1 L-cysteine chloride competitive Urtica dioica
1.14.18.1 salicylic acid uncompetitive Urtica dioica
1.14.18.1 Sodium azide noncompetitive Urtica dioica
1.14.18.1 Sodium cyanide noncompetitive Urtica dioica
1.14.18.1 Sodium diethyl dithiocarbamate cstrong competitive inhibitor Urtica dioica
1.14.18.1 Sodium metabisulfite competitive Urtica dioica
1.14.18.1 Thiourea competitive Urtica dioica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 0.414
-
 catechol pH 4.5, 25°C Urtica dioica
1.10.3.1 0.99
-
 (+)-catechin pH 4.0, 25°C Urtica dioica
1.10.3.1 1
-
 1,2,3-benzenetriol pH 3.5, 25°C Urtica dioica
1.10.3.1 1.25
-
 4-methylcatechol pH 3.5, 25°C Urtica dioica
1.10.3.1 21.4
-
 L-Dopa pH 3.5, 25°C Urtica dioica
1.14.18.1 0.54
-
 trans-cinnamic acid pH 3.5, 25°C Urtica dioica
1.14.18.1 0.79
-
 L-tyrosine pH 4.5, 25°C Urtica dioica
1.14.18.1 21.4
-
 L-Dopa pH 3.5, 25°C Urtica dioica

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Urtica dioica
-
 two isozymes
-
1.14.18.1 Urtica dioica
-
 two isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 native enzyme 17.8fold by (NH4)2SO4 precipitation, dialysis, and ion exchange chromatography Urtica dioica
1.14.18.1 native enzyme 17.8fold by (NH4)2SO4 precipitation, dialysis, and ion exchange chromatography Urtica dioica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 42.57
-
 purified enzyme Urtica dioica
1.14.18.1 42.57
-
 purified enzyme Urtica dioica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 (+)-catechin + O2
-
 Urtica dioica ?
-
 ?
1.10.3.1 4-methylcatechol + O2
-
 Urtica dioica 4-methyl-1,2-benzoquinone + H2O
-
 ?
1.10.3.1 catechol + 1/2 O2
-
 Urtica dioica 1,2-benzoquinone + H2O
-
 ?
1.10.3.1 L-dopa + 1/2 O2 i.e. L-3,4-dihydroxyphenylalanine Urtica dioica L-dopaquinone + H2O
-
 ?
1.10.3.1 additional information substrate specificity, overview, activity with phenolic and diphenolic substrates, also performing the reaction of tyrosinase, a ortho-hydroxylation of monophenols, EC 1.14.18.1, and the oxidation of catechols to ortho-quinones, the diphenolase activity, EC 1.10.3.1, overview Urtica dioica ?
-
 ?
1.10.3.1 pyrogallol + O2 low activity Urtica dioica ?
-
 ?
1.14.18.1 4-cresol + O2
-
 Urtica dioica ?
-
 ?
1.14.18.1 L-tyrosine + L-dopa + O2 high activity, best substrate Urtica dioica L-dopa + dopaquinone + H2O
-
 ?
1.14.18.1 additional information substrate specificity, overview, activity with phenolic and diphenolic substrates, also performing the reaction of tyrosinase, a ortho-hydroxylation of monophenols, EC 1.14.18.1, and the oxidation of catechols to ortho-quinones, the diphenolase activity, EC 1.10.3.1, overview Urtica dioica ?
-
 ?
1.14.18.1 trans-cinnamic acid + O2
-
 Urtica dioica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 More cf. EC 1.14.18.1 Urtica dioica
1.10.3.1 polyphenol oxidase
-
 Urtica dioica
1.10.3.1 PPO
-
 Urtica dioica
1.14.18.1 More cf. EC 1.10.3.1 Urtica dioica
1.14.18.1 polyphenol oxidase
-
 Urtica dioica
1.14.18.1 PPO
-
 Urtica dioica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 30 65 dependent on the substrate, overview Urtica dioica
1.14.18.1 30 65 dependent on the substrate, overview Urtica dioica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.1 5 80
-
 Urtica dioica
1.14.18.1 5 80
-
 Urtica dioica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 80
-
 purified enzyme, complete inactivation within 3 min Urtica dioica
1.14.18.1 80
-
 purified enzyme, complete inactivation within 3 min Urtica dioica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 3.5 4.5 dependent on the substrate, overview Urtica dioica
1.14.18.1 3.5 4.5 dependent on the substrate, overview Urtica dioica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.1 3 5.5
-
 Urtica dioica
1.14.18.1 3 5.5
-
 Urtica dioica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.18.1 0.00000179
-
 Sodium diethyl dithiocarbamate pH 6.5, 35°C, versus L-tyrosine Urtica dioica
1.14.18.1 0.00137
-
 L-cysteine chloride pH 6.5, 35°C, versus L-tyrosine Urtica dioica
1.14.18.1 0.0131
-
 benzoic acid pH 6.5, 35°C Urtica dioica
1.14.18.1 0.0164
-
 Sodium metabisulfite pH 6.5, 35°C, versus L-tyrosine Urtica dioica
1.14.18.1 0.0168
-
 L-ascorbic acid pH 6.5, 35°C, versus L-tyrosine Urtica dioica
1.14.18.1 0.074
-
 Sodium cyanide pH 6.5, 35°C Urtica dioica
1.14.18.1 0.084
-
 2-mercaptoethanol pH 6.5, 35°C, versus L-tyrosine Urtica dioica
1.14.18.1 0.213
-
 Thiourea pH 6.5, 35°C, versus L-tyrosine Urtica dioica
1.14.18.1 0.25
-
 Sodium azide pH 6.5, 35°C Urtica dioica
1.14.18.1 0.67
-
 salicylic acid pH 6.5, 35°C Urtica dioica