BRENDA - Enzyme Database

Purification and characterization of polyphenol oxidase from nettle (Urtica dioica L.) and inhibitory effects of some chemicals on enzyme activity

Guelcin, I.; Kuefrevioglu, O.I.; Oktay, M.; J. Enzyme Inhib. Med. Chem. 20, 297-302 (2005)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
2-mercaptoethanol
competitive
Urtica dioica
1.10.3.1
Benzoic acid
noncompetitive
Urtica dioica
1.10.3.1
glutathione
competitive
Urtica dioica
1.10.3.1
L-ascorbic acid
competitive
Urtica dioica
1.10.3.1
L-cysteine chloride
competitive
Urtica dioica
1.10.3.1
salicylic acid
uncompetitive
Urtica dioica
1.10.3.1
Sodium azide
noncompetitive
Urtica dioica
1.10.3.1
Sodium cyanide
noncompetitive
Urtica dioica
1.10.3.1
Sodium diethyl dithiocarbamate
cstrong competitive inhibitor
Urtica dioica
1.10.3.1
Sodium metabisulfite
competitive
Urtica dioica
1.10.3.1
Thiourea
competitive
Urtica dioica
1.14.18.1
2-mercaptoethanol
competitive
Urtica dioica
1.14.18.1
Benzoic acid
noncompetitive
Urtica dioica
1.14.18.1
glutathione
competitive
Urtica dioica
1.14.18.1
L-ascorbic acid
competitive
Urtica dioica
1.14.18.1
L-cysteine chloride
competitive
Urtica dioica
1.14.18.1
salicylic acid
uncompetitive
Urtica dioica
1.14.18.1
Sodium azide
noncompetitive
Urtica dioica
1.14.18.1
Sodium cyanide
noncompetitive
Urtica dioica
1.14.18.1
Sodium diethyl dithiocarbamate
cstrong competitive inhibitor
Urtica dioica
1.14.18.1
Sodium metabisulfite
competitive
Urtica dioica
1.14.18.1
Thiourea
competitive
Urtica dioica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.1
0.414
-
catechol
pH 4.5, 25°C
Urtica dioica
1.10.3.1
0.99
-
(+)-catechin
pH 4.0, 25°C
Urtica dioica
1.10.3.1
1
-
1,2,3-benzenetriol
pH 3.5, 25°C
Urtica dioica
1.10.3.1
1.25
-
4-methylcatechol
pH 3.5, 25°C
Urtica dioica
1.10.3.1
21.4
-
L-Dopa
pH 3.5, 25°C
Urtica dioica
1.14.18.1
0.54
-
trans-cinnamic acid
pH 3.5, 25°C
Urtica dioica
1.14.18.1
0.79
-
L-tyrosine
pH 4.5, 25°C
Urtica dioica
1.14.18.1
21.4
-
L-Dopa
pH 3.5, 25°C
Urtica dioica
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.10.3.1
Urtica dioica
-
two isozymes
-
1.14.18.1
Urtica dioica
-
two isozymes
-
Purification (Commentary)
EC Number
Commentary
Organism
1.10.3.1
native enzyme 17.8fold by (NH4)2SO4 precipitation, dialysis, and ion exchange chromatography
Urtica dioica
1.14.18.1
native enzyme 17.8fold by (NH4)2SO4 precipitation, dialysis, and ion exchange chromatography
Urtica dioica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.10.3.1
42.57
-
purified enzyme
Urtica dioica
1.14.18.1
42.57
-
purified enzyme
Urtica dioica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
(+)-catechin + O2
-
675101
Urtica dioica
?
-
-
-
?
1.10.3.1
4-methylcatechol + O2
-
675101
Urtica dioica
4-methyl-1,2-benzoquinone + H2O
-
-
-
?
1.10.3.1
catechol + 1/2 O2
-
675101
Urtica dioica
1,2-benzoquinone + H2O
-
-
-
?
1.10.3.1
L-dopa + 1/2 O2
i.e. L-3,4-dihydroxyphenylalanine
675101
Urtica dioica
L-dopaquinone + H2O
-
-
-
?
1.10.3.1
additional information
substrate specificity, overview, activity with phenolic and diphenolic substrates, also performing the reaction of tyrosinase, a ortho-hydroxylation of monophenols, EC 1.14.18.1, and the oxidation of catechols to ortho-quinones, the diphenolase activity, EC 1.10.3.1, overview
675101
Urtica dioica
?
-
-
-
-
1.10.3.1
pyrogallol + O2
low activity
675101
Urtica dioica
?
-
-
-
?
1.14.18.1
4-cresol + O2
-
675101
Urtica dioica
?
-
-
-
?
1.14.18.1
L-tyrosine + L-dopa + O2
high activity, best substrate
675101
Urtica dioica
L-dopa + dopaquinone + H2O
-
-
-
-
1.14.18.1
additional information
substrate specificity, overview, activity with phenolic and diphenolic substrates, also performing the reaction of tyrosinase, a ortho-hydroxylation of monophenols, EC 1.14.18.1, and the oxidation of catechols to ortho-quinones, the diphenolase activity, EC 1.10.3.1, overview
675101
Urtica dioica
?
-
-
-
-
1.14.18.1
trans-cinnamic acid + O2
-
675101
Urtica dioica
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.1
30
65
dependent on the substrate, overview
Urtica dioica
1.14.18.1
30
65
dependent on the substrate, overview
Urtica dioica
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.10.3.1
5
80
-
Urtica dioica
1.14.18.1
5
80
-
Urtica dioica
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.10.3.1
80
-
purified enzyme, complete inactivation within 3 min
Urtica dioica
1.14.18.1
80
-
purified enzyme, complete inactivation within 3 min
Urtica dioica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.1
3.5
4.5
dependent on the substrate, overview
Urtica dioica
1.14.18.1
3.5
4.5
dependent on the substrate, overview
Urtica dioica
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.10.3.1
3
5.5
-
Urtica dioica
1.14.18.1
3
5.5
-
Urtica dioica
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.18.1
0.00000179
-
Sodium diethyl dithiocarbamate
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.00137
-
L-cysteine chloride
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.0131
-
Benzoic acid
pH 6.5, 35°C
Urtica dioica
1.14.18.1
0.0164
-
Sodium metabisulfite
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.0168
-
L-ascorbic acid
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.074
-
Sodium cyanide
pH 6.5, 35°C
Urtica dioica
1.14.18.1
0.084
-
2-mercaptoethanol
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.213
-
Thiourea
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.25
-
Sodium azide
pH 6.5, 35°C
Urtica dioica
1.14.18.1
0.67
-
salicylic acid
pH 6.5, 35°C
Urtica dioica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
2-mercaptoethanol
competitive
Urtica dioica
1.10.3.1
Benzoic acid
noncompetitive
Urtica dioica
1.10.3.1
glutathione
competitive
Urtica dioica
1.10.3.1
L-ascorbic acid
competitive
Urtica dioica
1.10.3.1
L-cysteine chloride
competitive
Urtica dioica
1.10.3.1
salicylic acid
uncompetitive
Urtica dioica
1.10.3.1
Sodium azide
noncompetitive
Urtica dioica
1.10.3.1
Sodium cyanide
noncompetitive
Urtica dioica
1.10.3.1
Sodium diethyl dithiocarbamate
cstrong competitive inhibitor
Urtica dioica
1.10.3.1
Sodium metabisulfite
competitive
Urtica dioica
1.10.3.1
Thiourea
competitive
Urtica dioica
1.14.18.1
2-mercaptoethanol
competitive
Urtica dioica
1.14.18.1
Benzoic acid
noncompetitive
Urtica dioica
1.14.18.1
glutathione
competitive
Urtica dioica
1.14.18.1
L-ascorbic acid
competitive
Urtica dioica
1.14.18.1
L-cysteine chloride
competitive
Urtica dioica
1.14.18.1
salicylic acid
uncompetitive
Urtica dioica
1.14.18.1
Sodium azide
noncompetitive
Urtica dioica
1.14.18.1
Sodium cyanide
noncompetitive
Urtica dioica
1.14.18.1
Sodium diethyl dithiocarbamate
cstrong competitive inhibitor
Urtica dioica
1.14.18.1
Sodium metabisulfite
competitive
Urtica dioica
1.14.18.1
Thiourea
competitive
Urtica dioica
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.18.1
0.00000179
-
Sodium diethyl dithiocarbamate
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.00137
-
L-cysteine chloride
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.0131
-
Benzoic acid
pH 6.5, 35°C
Urtica dioica
1.14.18.1
0.0164
-
Sodium metabisulfite
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.0168
-
L-ascorbic acid
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.074
-
Sodium cyanide
pH 6.5, 35°C
Urtica dioica
1.14.18.1
0.084
-
2-mercaptoethanol
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.213
-
Thiourea
pH 6.5, 35°C, versus L-tyrosine
Urtica dioica
1.14.18.1
0.25
-
Sodium azide
pH 6.5, 35°C
Urtica dioica
1.14.18.1
0.67
-
salicylic acid
pH 6.5, 35°C
Urtica dioica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.1
0.414
-
catechol
pH 4.5, 25°C
Urtica dioica
1.10.3.1
0.99
-
(+)-catechin
pH 4.0, 25°C
Urtica dioica
1.10.3.1
1
-
1,2,3-benzenetriol
pH 3.5, 25°C
Urtica dioica
1.10.3.1
1.25
-
4-methylcatechol
pH 3.5, 25°C
Urtica dioica
1.10.3.1
21.4
-
L-Dopa
pH 3.5, 25°C
Urtica dioica
1.14.18.1
0.54
-
trans-cinnamic acid
pH 3.5, 25°C
Urtica dioica
1.14.18.1
0.79
-
L-tyrosine
pH 4.5, 25°C
Urtica dioica
1.14.18.1
21.4
-
L-Dopa
pH 3.5, 25°C
Urtica dioica
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.10.3.1
native enzyme 17.8fold by (NH4)2SO4 precipitation, dialysis, and ion exchange chromatography
Urtica dioica
1.14.18.1
native enzyme 17.8fold by (NH4)2SO4 precipitation, dialysis, and ion exchange chromatography
Urtica dioica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.10.3.1
42.57
-
purified enzyme
Urtica dioica
1.14.18.1
42.57
-
purified enzyme
Urtica dioica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
(+)-catechin + O2
-
675101
Urtica dioica
?
-
-
-
?
1.10.3.1
4-methylcatechol + O2
-
675101
Urtica dioica
4-methyl-1,2-benzoquinone + H2O
-
-
-
?
1.10.3.1
catechol + 1/2 O2
-
675101
Urtica dioica
1,2-benzoquinone + H2O
-
-
-
?
1.10.3.1
L-dopa + 1/2 O2
i.e. L-3,4-dihydroxyphenylalanine
675101
Urtica dioica
L-dopaquinone + H2O
-
-
-
?
1.10.3.1
additional information
substrate specificity, overview, activity with phenolic and diphenolic substrates, also performing the reaction of tyrosinase, a ortho-hydroxylation of monophenols, EC 1.14.18.1, and the oxidation of catechols to ortho-quinones, the diphenolase activity, EC 1.10.3.1, overview
675101
Urtica dioica
?
-
-
-
-
1.10.3.1
pyrogallol + O2
low activity
675101
Urtica dioica
?
-
-
-
?
1.14.18.1
4-cresol + O2
-
675101
Urtica dioica
?
-
-
-
?
1.14.18.1
L-tyrosine + L-dopa + O2
high activity, best substrate
675101
Urtica dioica
L-dopa + dopaquinone + H2O
-
-
-
-
1.14.18.1
additional information
substrate specificity, overview, activity with phenolic and diphenolic substrates, also performing the reaction of tyrosinase, a ortho-hydroxylation of monophenols, EC 1.14.18.1, and the oxidation of catechols to ortho-quinones, the diphenolase activity, EC 1.10.3.1, overview
675101
Urtica dioica
?
-
-
-
-
1.14.18.1
trans-cinnamic acid + O2
-
675101
Urtica dioica
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.1
30
65
dependent on the substrate, overview
Urtica dioica
1.14.18.1
30
65
dependent on the substrate, overview
Urtica dioica
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.10.3.1
5
80
-
Urtica dioica
1.14.18.1
5
80
-
Urtica dioica
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.10.3.1
80
-
purified enzyme, complete inactivation within 3 min
Urtica dioica
1.14.18.1
80
-
purified enzyme, complete inactivation within 3 min
Urtica dioica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.1
3.5
4.5
dependent on the substrate, overview
Urtica dioica
1.14.18.1
3.5
4.5
dependent on the substrate, overview
Urtica dioica
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.10.3.1
3
5.5
-
Urtica dioica
1.14.18.1
3
5.5
-
Urtica dioica