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Literature summary extracted from

  • Ko, H.S.; Yokoyama, Y.; Ohno, N.; Okadome, M.; Amachi, S.; Shinoyama, H.; Fujii, T.
    Purification and characterization of intracellular and extracellular, thermostable and alkali-tolerant alcohol oxidases produced by a thermophilic fungus, Thermoascus aurantiacus NBRC 31693 (2005), J. Biosci. Bioeng., 99, 348-353.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 EDTA slight inhibition of intra- and extracellular enzymes Thermoascus aurantiacus
1.1.3.13 Fe2+ slight inhibition of extracellular enzyme Thermoascus aurantiacus
1.1.3.13 Hg2+ strong inhibition of intra- and extracellular enzymes Thermoascus aurantiacus
1.1.3.13 K+ slight inhibition of extracellular enzyme Thermoascus aurantiacus
1.1.3.13 KCN inhibition of intra- and extracellular enzymes Thermoascus aurantiacus
1.1.3.13 Mn2+ slight inhibition of intra- and extracellular enzymes Thermoascus aurantiacus
1.1.3.13 Na+ slight inhibition of extracellular enzyme Thermoascus aurantiacus
1.1.3.13 NaN3 inhibition of intra- and extracellular enzymes Thermoascus aurantiacus
1.1.3.13 NEM slight inhibition of intra- and extracellular enzymes Thermoascus aurantiacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 0.5
-
 methanol extracellular enzyme, pH 5.9, 30°C Thermoascus aurantiacus
1.1.3.13 10.2
-
 ethanol extracellular enzyme, pH 5.9, 30°C Thermoascus aurantiacus
1.1.3.13 13.5
-
 methanol intracellular enzyme, pH 5.9, 30°C Thermoascus aurantiacus
1.1.3.13 15.8
-
 ethanol intracellular enzyme, pH 5.9, 30°C Thermoascus aurantiacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.13 K+ slight activation of intracellular enzyme Thermoascus aurantiacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 320000
-
 intracellular enzyme, gel filtration Thermoascus aurantiacus
1.1.3.13 560000
-
 extracellular enzyme, gel filtration Thermoascus aurantiacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.13 ethanol + O2 Thermoascus aurantiacus
-
 acetaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 Thermoascus aurantiacus
-
 formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 Thermoascus aurantiacus NBRC 31693
-
 formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Thermoascus aurantiacus
-
 thermophilic fungus
-
1.1.3.13 Thermoascus aurantiacus NBRC 31693
-
 thermophilic fungus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13 native intracellular enzyme, 9.24fold, and extracellular enzyme, 314fol, from strain NBRC 31693, by streptomycin precipitation, dialysis, anion exchange chromatography and gel filtration or by polethylene glycol precipitation, anion exchange and hydrophobic interaction chromatography, and gel filtration, respectively Thermoascus aurantiacus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 1.67
-
 purified intracellular enzyme Thermoascus aurantiacus
1.1.3.13 9.42
-
 purified extracellular enzyme Thermoascus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 1-butanol + O2 intra- and extracellular enzymes, low activity with the intracellular enzyme Thermoascus aurantiacus butanaldehyde + H2O2
-
 ?
1.1.3.13 1-pentanol + O2 intra- and extracellular enzymes, low activity with the intracellular enzyme Thermoascus aurantiacus pentanaldehyde + H2O2
-
 ?
1.1.3.13 2-methyl-2-propanol + O2 extracellular enzyme, no activity with the intracellular enzyme Thermoascus aurantiacus ?
-
 ?
1.1.3.13 2-propanol + O2 low activity with the extracellular enzyme Thermoascus aurantiacus acetone + H2O2
-
 ?
1.1.3.13 2-propanol + O2 low activity with the extracellular enzyme Thermoascus aurantiacus NBRC 31693 acetone + H2O2
-
 ?
1.1.3.13 arabitol + O2 extracellular enzyme, no activity with the intracellular enzyme Thermoascus aurantiacus ?
-
 ?
1.1.3.13 benzyl alcohol + O2 extracellular enzyme, no activity with the intracellular enzyme Thermoascus aurantiacus benzaldehyde + H2O2
-
 ?
1.1.3.13 benzyl alcohol + O2 extracellular enzyme, no activity with the intracellular enzyme Thermoascus aurantiacus NBRC 31693 benzaldehyde + H2O2
-
 ?
1.1.3.13 ethanol + O2
-
 Thermoascus aurantiacus acetaldehyde + H2O2
-
 ?
1.1.3.13 ethanol + O2 intra- and extracellular enzymes Thermoascus aurantiacus acetaldehyde + H2O2
-
 ?
1.1.3.13 ethylene glycol + O2 intra- and extracellular enzymes, low activity with the intracellular enzyme Thermoascus aurantiacus ?
-
 ?
1.1.3.13 methanol + O2
-
 Thermoascus aurantiacus formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 intra- and extracellular enzymes Thermoascus aurantiacus formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Thermoascus aurantiacus NBRC 31693 formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 intra- and extracellular enzymes Thermoascus aurantiacus NBRC 31693 formaldehyde + H2O2
-
 ?
1.1.3.13 additional information substrate specificities of intra- and extracellular enzymes, overview, no activity by both with 2-butanol, 2-methyl-1-propanol, and glycerol Thermoascus aurantiacus ?
-
 ?
1.1.3.13 additional information substrate specificities of intra- and extracellular enzymes, overview, no activity by both with 2-butanol, 2-methyl-1-propanol, and glycerol Thermoascus aurantiacus NBRC 31693 ?
-
 ?
1.1.3.13 n-propanol + O2 intra- and extracellular enzymes Thermoascus aurantiacus propionaldehyde + H2O2
-
 ?
1.1.3.13 propylene glycol + O2 low activity with intra- and extracellular enzymes Thermoascus aurantiacus ?
-
 ?
1.1.3.13 veratryl alcohol + O2 extracellular enzyme, no activity with the intracellular enzyme Thermoascus aurantiacus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.13 AOext
-
 Thermoascus aurantiacus
1.1.3.13 AOint
-
 Thermoascus aurantiacus
1.1.3.13 extracellular alcohol oxidase
-
 Thermoascus aurantiacus
1.1.3.13 intracellular alcohol oxidase
-
 Thermoascus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.13 30
-
 assay at Thermoascus aurantiacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.13 55
-
 15 min, pH 8.0, purified extracellular enzyme, completely stable Thermoascus aurantiacus
1.1.3.13 60
-
 15 min, pH 8.0, purified intracellular enzyme, completely stable Thermoascus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 7.2
-
 assay at Thermoascus aurantiacus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.13 5 11 purified intracellular enzyme, stable Thermoascus aurantiacus
1.1.3.13 6 11 purified extracellular enzyme, stable Thermoascus aurantiacus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.13 Thermoascus aurantiacus purified intracellular enzyme
-
 5.9
1.1.3.13 Thermoascus aurantiacus purified extracellular enzyme
-
 6.1