EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.22 | expression in Escherichia coli BL21(DE3)pLysS as His-tag fusion protein | Escherichia coli |
1.14.15.1 | individual expression of enzyme and cofactor putidaredoxin in Escherichia coli | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.15.1 | additional information | establishment of a functional CYP101-system in a cell-like aqueous compartment build by a stable water-oil emulsion with the non-ioninc surfactant tetraethylene glycol dodecyl ether in micro-scale, the enzyme is not active in an organic-aqueous biphasic system, e.g. with etahnol or glycol, incorporating an NADH-regeneration system using recombinant His-tagged bacterial glycerol dehydrogenase, method optimization, overview | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin | Pseudomonas putida | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.22 | Escherichia coli | - |
- |
- |
1.1.99.22 | Escherichia coli JM109 | - |
- |
- |
1.14.15.1 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.22 | to homogeneity by anion exchange chromatography and Ni-affinity chromatography | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.22 | glycerol + NAD+ | - |
Escherichia coli | dihydroxyacetone + NADH + H+ | - |
? | |
1.1.99.22 | glycerol + NAD+ | - |
Escherichia coli JM109 | dihydroxyacetone + NADH + H+ | - |
? | |
1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin | - |
Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
1.14.15.1 | (+)-camphor + O2 + reduced putidaredoxin | mechanism of camphor hydroxylation incorporating an NADH-regeneration system, overview | Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.22 | GLD | - |
Escherichia coli |
1.1.99.22 | glycerol dehydrogenase | - |
Escherichia coli |
1.14.15.1 | CYP101 | - |
Pseudomonas putida |
1.14.15.1 | cytochrome P450cam monooxygenase | - |
Pseudomonas putida |
1.14.15.1 | P450cam | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 37 | - |
assay at | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 7.4 | - |
- |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.1 | putidaredoxin | - |
Pseudomonas putida |