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Literature summary extracted from

  • Sato, M.; Masuda, Y.; Kirimura, K.; Kino, K.
    Thermostable ATP regeneration system using polyphosphate kinase from Thermosynechococcus elongatus BP-1 for D-amino acid dipeptide synthesis (2007), J. Biosci. Bioeng., 103, 179-184.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.1 (NH4)2SO4 not activating Thermosynechococcus vestitus
2.7.4.1 additional information (NH4)2SO4 is not activating Mycobacterium tuberculosis

Application

EC Number Application Comment Organism
2.7.4.1 biotechnology ATP supply for synthesis of D-amino acid dipeptides Thermosynechococcus vestitus
2.7.4.1 biotechnology ATP supply for synthesis of D-amino acid dipeptides Mycobacterium tuberculosis
2.7.4.1 synthesis the enzyme is used to establih a thermostable ATP regeneration system from polyphosphate substrate, this is then used for synthesis of D-Ala-D-Ala in a coupled system with the thermostable D-alanine-D-alanine ligase TmDdl from Thermotoga maritima, a useful biocatalyst for synthesizing D-amino acid dipeptides, method development and optimization, overview Thermosynechococcus vestitus
6.3.2.4 synthesis D-alanine-D-alanine ligase is a biocatalyst for synthesizing D-amino acid dipeptides, use of a thermostable ATP regeneration system Thermosynechococcus vestitus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1 expression of His6-tagged Ppk in Escherichia coli strain BL21(DE3) Thermosynechococcus vestitus
2.7.4.1 overexpression in Escherichia coli Thermosynechococcus vestitus
2.7.4.1 overexpression in Escherichia coli Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+
-
 Thermosynechococcus vestitus
2.7.4.1 Mg2+ optimal above 0.5 mM Thermosynechococcus vestitus
2.7.4.1 Mg2+ optimal above 0.5 mM Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.1 ATP + (phosphate)n Thermosynechococcus vestitus
-
 ADP + (phosphate)n+1
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Mycobacterium tuberculosis P9WHV9
-
-
2.7.4.1 Mycobacterium tuberculosis H37Rv P9WHV9
-
-
2.7.4.1 Thermosynechococcus vestitus
-
 BP-1
-
2.7.4.1 Thermosynechococcus vestitus Q8DMA8
-
-
6.3.2.4 Thermosynechococcus vestitus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1 of the recombinant His x6-tagged protein Thermosynechococcus vestitus
2.7.4.1 of the recombinant His x6-tagged protein Mycobacterium tuberculosis
2.7.4.1 recombinant His6-tagged Ppk 21fold from Escherichia coli strain BL21(DE3) by His-Trap chromatography Thermosynechococcus vestitus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.1
-
-
 of the purified protein Thermosynechococcus vestitus
2.7.4.1 0.317
-
 purified recombinant His-tagged Ppk Thermosynechococcus vestitus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ADP + (phosphate)n not (phosphate)3, (phosphate)4 as preferred phosphate donor Thermosynechococcus vestitus ATP + (phosphate)n-1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Mycobacterium tuberculosis ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Thermosynechococcus vestitus ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Mycobacterium tuberculosis H37Rv ADP + (phosphate)n+1
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.4.1 dimer
-
 Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 ATP:polyphosphate phosphotransferase
-
 Thermosynechococcus vestitus
2.7.4.1 ATP:polyphosphate phosphotransferase
-
 Mycobacterium tuberculosis
2.7.4.1 kinase, polyphosphate (phosphorylating)
-
 Thermosynechococcus vestitus
2.7.4.1 kinase, polyphosphate (phosphorylating)
-
 Mycobacterium tuberculosis
2.7.4.1 polyphosphate kinase
-
 Thermosynechococcus vestitus
2.7.4.1 polyphosphate kinase
-
 Mycobacterium tuberculosis
2.7.4.1 PPK
-
 Thermosynechococcus vestitus
2.7.4.1 PPK
-
 Mycobacterium tuberculosis
2.7.4.1 PPK2
-
 Thermosynechococcus vestitus
2.7.4.1 PPK2B
-
 Thermosynechococcus vestitus
6.3.2.4 TmDdl
-
 Thermosynechococcus vestitus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.1 60
-
-
 Thermosynechococcus vestitus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.4.1 30 75
-
 Thermosynechococcus vestitus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.1 70
-
 stable up to Thermosynechococcus vestitus
6.3.2.4 70
-
 stable at Thermosynechococcus vestitus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 8
-
-
 Thermosynechococcus vestitus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.1 6 9
-
 Thermosynechococcus vestitus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.1 ATP
-
 Thermosynechococcus vestitus