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Literature summary extracted from

  • Menavuvu, B.T.; Poonperm, W.; Takeda, K.; Morimoto, K.; Granstroem, T.B.; Takada, G.; Izumori, K.
    Novel substrate specificity of D-arabinose isomerase from Klebsiella pneumoniae and its application to production of D-altrose from D-psicose (2006), J. Biosci. Bioeng., 102, 436-441.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.3 60000
-
 SDS-PAGE Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.3 Klebsiella pneumoniae
-
 40bXX
-
5.3.1.3 Klebsiella pneumoniae 40bXX
-
 40bXX
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.3 of the wild type protein by Q-sepharose HP and Phenyl-sepharose column chromatography Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.3 0.73
-
 after purification, L-galactose as substrate Klebsiella pneumoniae
5.3.1.3 1
-
 after purification, D-altrose as substrate Klebsiella pneumoniae
5.3.1.3 1
-
 after purification, D-xylose as substrate Klebsiella pneumoniae
5.3.1.3 1
-
 after purification, L-ribose as substrate Klebsiella pneumoniae
5.3.1.3 1.3
-
 after purification, D-galactose as substrate Klebsiella pneumoniae
5.3.1.3 1.3
-
 after purification, D-lyxose as substrate Klebsiella pneumoniae
5.3.1.3 1.9
-
 after purification, L-glucose as substrate Klebsiella pneumoniae
5.3.1.3 2.7
-
 after purification, L-lyxose as substrate Klebsiella pneumoniae
5.3.1.3 2.8
-
 after purification, D-mannose as substrate Klebsiella pneumoniae
5.3.1.3 3 8 after purification, L-fucose as substrate Klebsiella pneumoniae
5.3.1.3 4.7
-
 after purification, L-xylose as substrate Klebsiella pneumoniae
5.3.1.3 36.5
-
 after purification, D-arabinose as substrate Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.3 D-altrose
-
 Klebsiella pneumoniae D-psicose
-
 r
5.3.1.3 D-arabinose
-
 Klebsiella pneumoniae D-ribulose
-
 r
5.3.1.3 D-arabinose
-
 Klebsiella pneumoniae 40bXX D-ribulose
-
 r
5.3.1.3 D-galactose
-
 Klebsiella pneumoniae D-tagatose
-
 r
5.3.1.3 D-Lyxose
-
 Klebsiella pneumoniae D-Xylulose
-
 r
5.3.1.3 D-Lyxose
-
 Klebsiella pneumoniae 40bXX D-Xylulose
-
 r
5.3.1.3 D-Mannose
-
 Klebsiella pneumoniae D-Fructose
-
 r
5.3.1.3 D-Mannose
-
 Klebsiella pneumoniae 40bXX D-Fructose
-
 r
5.3.1.3 D-Xylose
-
 Klebsiella pneumoniae D-Xylulose
-
 r
5.3.1.3 L-Fucose
-
 Klebsiella pneumoniae L-Fuculose
-
 r
5.3.1.3 L-galactose
-
 Klebsiella pneumoniae L-tagatose
-
 r
5.3.1.3 L-glucose
-
 Klebsiella pneumoniae L-fructose
-
 r
5.3.1.3 L-lyxose
-
 Klebsiella pneumoniae L-xylulose
-
 r
5.3.1.3 L-lyxose
-
 Klebsiella pneumoniae 40bXX L-xylulose
-
 r
5.3.1.3 L-ribose
-
 Klebsiella pneumoniae L-ribulose
-
 r
5.3.1.3 L-Xylose
-
 Klebsiella pneumoniae L-Xylulose
-
 r
5.3.1.3 L-Xylose
-
 Klebsiella pneumoniae 40bXX L-Xylulose
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.3.1.3 D-arabinose aldose-ketose-isomerase
-
 Klebsiella pneumoniae
5.3.1.3 D-Arabinose isomerase D-AI Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.3 40
-
-
 Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.3 9
-
-
 Klebsiella pneumoniae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.3 9
-
-
 Klebsiella pneumoniae