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Literature summary extracted from

  • Valente, F.M.; Oliveira, A.S.; Gnadt, N.; Pacheco, I.; Coelho, A.V.; Xavier, A.V.; Teixeira, M.; Soares, C.M.; Pereira, I.A.
    Hydrogenases in Desulfovibrio vulgaris Hildenborough: structural and physiologic characterisation of the membrane-bound [NiFeSe] hydrogenase (2005), J. Biol. Inorg. Chem., 10, 667-682.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.2.1 Phospholipid H2-production from reduced methyl viologen requires phospholipids Desulfovibrio vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.2.1 cytoplasmic membrane
-
 Desulfovibrio vulgaris
-
-
1.12.2.1 cytoplasmic membrane of lactate/sulfate grown cells. The enzyme is present in the cell in two states: the majority is membrane-associated (the [NiFeSe]m form) and a minor amount is soluble (the [NiFeSe]s form) Desulfovibrio vulgaris
-
-
1.12.2.1 soluble of lactate/sulfate grown cells. The enzyme is present in the cell in two states: the majority is membrane-associated (the [NiFeSe]m form) and a minor amount is soluble (the [NiFeSe]s form) Desulfovibrio vulgaris
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.2.1 Fe NiFeSe enzyme Desulfovibrio vulgaris
1.12.2.1 Fe [NiFeSe] enzyme, ratio Ni:Se:Fe is 1:1:15 Desulfovibrio vulgaris
1.12.2.1 Ni NiFeSe enzyme, contains selenium in equimolar amounts to Ni Desulfovibrio vulgaris
1.12.2.1 Ni [NiFeSe] enzyme, ratio Ni:Se:Fe is 1:1:15 Desulfovibrio vulgaris
1.12.2.1 Se NiFeSe enzyme, contains selenium in equimolar amounts to Ni Desulfovibrio vulgaris
1.12.2.1 Se [NiFeSe] enzyme, ratio Ni:Se:Fe is 1:1:15 Desulfovibrio vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.2.1 35000
-
 x * 63000 + x * 35000, SDS-PAGE Desulfovibrio vulgaris
1.12.2.1 63000
-
 x * 63000 + x * 35000, SDS-PAGE Desulfovibrio vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.2.1 H2 + ferricytochrome c3 Desulfovibrio vulgaris
-
 H+ + ferrocytochrome c3
-
 ?
1.12.2.1 H2 + ferricytochrome c3 Desulfovibrio vulgaris Hildenborough
-
 H+ + ferrocytochrome c3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.2.1 Desulfovibrio vulgaris
-
-
-
1.12.2.1 Desulfovibrio vulgaris
-
 Hildenborough
-
1.12.2.1 Desulfovibrio vulgaris Hildenborough
-
-
-
1.12.2.1 Desulfovibrio vulgaris Hildenborough
-
 Hildenborough
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.2.1 resistance of the [NiFeSe] Hase to inactivation by oxygen Desulfovibrio vulgaris

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.12.2.1 lipoprotein post-translationally modified, probably by the binding of a lipidic group at the N-terminus. The presence of such a group explains the membrane association of the enzyme, the requirement of phospholipids for maximal activity, and the blockage of the large subunit to N-terminal sequencing Desulfovibrio vulgaris

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.2.1
-
 Desulfovibrio vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.12.2.1 culture condition:lactate/sulfate-grown cell
-
 Desulfovibrio vulgaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.2.1 H+ + ferrocytochrome c3
-
 Desulfovibrio vulgaris H2 + ferricytochrome c3
-
 ?
1.12.2.1 H+ + ferrocytochrome c3
-
 Desulfovibrio vulgaris Hildenborough H2 + ferricytochrome c3
-
 ?
1.12.2.1 H+ + reduced methyl viologen
-
 Desulfovibrio vulgaris H2 + methyl viologen
-
 ?
1.12.2.1 H+ + reduced methyl viologen
-
 Desulfovibrio vulgaris Hildenborough H2 + methyl viologen
-
 ?
1.12.2.1 H2 + ferricytochrome c
-
 Desulfovibrio vulgaris H+ + ferrocytochrome c
-
 ?
1.12.2.1 H2 + ferricytochrome c
-
 Desulfovibrio vulgaris Hildenborough H+ + ferrocytochrome c
-
 ?
1.12.2.1 H2 + ferricytochrome c3
-
 Desulfovibrio vulgaris H+ + ferrocytochrome c3
-
 ?
1.12.2.1 H2 + ferricytochrome c3
-
 Desulfovibrio vulgaris Hildenborough H+ + ferrocytochrome c3
-
 ?
1.12.2.1 reduced methyl viologen + H+
-
 Desulfovibrio vulgaris oxidized methyl viologen + H2
-
 ?
1.12.2.1 reduced methyl viologen + H+
-
 Desulfovibrio vulgaris Hildenborough oxidized methyl viologen + H2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.2.1 ? x * 63000 + x * 35000, SDS-PAGE Desulfovibrio vulgaris

Synonyms

EC Number Synonyms Comment Organism
1.12.2.1 [NiFeSe] Hase
-
 Desulfovibrio vulgaris
1.12.2.1 [NiFeSe] hydrogenase
-
 Desulfovibrio vulgaris